人类超氧化物歧化酶中的荧光寿命分布。温度和变性的影响。
Fluorescence lifetime distributions in human superoxide dismutase. Effect of temperature and denaturation.
作者信息
Rosato N, Gratton E, Mei G, Finazzi-Agrò A
机构信息
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Roma Tor Vergata, Italy.
出版信息
Biophys J. 1990 Oct;58(4):817-22. doi: 10.1016/S0006-3495(90)82427-9.
Abstract
The internal dynamics of human superoxide dismutase has been studied using time-resolved fluorescence. The fluorescence decay has been analyzed using continuous distribution of lifetime values. The effect of temperature and conformational state on the lifetime distribution has been investigated. The emission of the single tryptophan residue depends on the nature and dynamics of the protein matrix. Conformational changes have been induced by increased concentration of guanidinium hydrochloride. We found that both temperature and conformation strongly effect the width of the lifetime distribution.
摘要
已使用时间分辨荧光研究了人类超氧化物歧化酶的内部动力学。利用寿命值的连续分布对荧光衰减进行了分析。研究了温度和构象状态对寿命分布的影响。单个色氨酸残基的发射取决于蛋白质基质的性质和动力学。通过增加盐酸胍的浓度诱导了构象变化。我们发现温度和构象都强烈影响寿命分布的宽度。
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