一种具有皮秒分辨率的连续可变频率互相关相位荧光计。
A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution.
作者信息
Gratton E, Limkeman M
出版信息
Biophys J. 1983 Dec;44(3):315-24. doi: 10.1016/S0006-3495(83)84305-7.
Abstract
A detailed description of the construction and performance of a variable frequency cross-correlation phase fluorometer is reported. The phase fluorometer operates over the frequency range 1-160 MHz with a maximum resolution of a few picoseconds. The effects of distortions introduced by the light modulator and the nonlinear dynode characteristic are discussed in terms of the harmonic content of the detected signal. A source of systematic errors due to nonhomogeneous modulation is also discussed with particular attention to the color effect of the photomultipliers. The application of the phase fluorometer to the measurement of very long and very short lifetimes is reported. Some application to the measurement of multiexponential decays is also illustrated.
摘要
本文报道了一种可变频率互相关相位荧光计的构造和性能的详细描述。该相位荧光计在1 - 160 MHz的频率范围内工作，最大分辨率可达几皮秒。根据检测信号的谐波含量，讨论了光调制器引入的失真和非线性倍增极特性的影响。还讨论了由于调制不均匀导致的系统误差源，特别关注了光电倍增管的颜色效应。报道了该相位荧光计在测量极长和极短寿命方面的应用。还举例说明了其在多指数衰减测量中的一些应用。
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