Li Na, Wen Zhan-bo, Yang Wen-hui, Wang Jie, Li Jin-song, Hu Ling-fei, Dong Xiao-kai, Liu Ke-yang, Cao Jie
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, State Key Laboratory of Pathogen and Biosecurity, National Research Center of Bio-Protection Equipment and Engineering, Beijing 100071, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2012 Jan;46(1):67-9.
To evaluate the protective performance of a positive pressure bio-protective clothing against viral aerosol.
The suspension of indicating virus phage Phi-X174 was made for viral aerosol generating in a hermetic cabin. The diameter of viral aerosol particles were measured with a aerodynamics size analyzer. By adjusting the inner humidity of the cabin, the protective efficiency of the positive pressure bio-protective clothing against viral aerosol in high and low windshield conditions was determined with Andersen six-stage air sampler sampling and plage forming unit (PFU) counting, respectively.
The mass median diameter of Phage Phi-X174 aerosol particles was about 0.922 µm and the background concentration is beyond 2 × 10⁴ particles/m³. The protective efficiency of the clothing against phage Phi-X174 aerosol particles was above 99.9% under different test conditions with the range of viral aerosol concentration between 0 - 23 PFU/m³. Airflow (P = 0.84), environment humidity conditions (P = 0.33) and sampling time (P = 0.07) did not affect the protective efficiency statistically.
The positive pressure bio-protective clothing provided a relatively high efficiency against phage Phi-X174 aerosol regardless of airflow rate, environment humidity and sampling time.
评估一种正压生物防护服对病毒气溶胶的防护性能。
制备指示病毒噬菌体Phi-X174的悬液用于在密闭舱内产生病毒气溶胶。用空气动力学粒径分析仪测量病毒气溶胶颗粒的直径。通过调节舱内湿度,分别采用安德森六级空气采样器采样和蚀斑形成单位(PFU)计数法,测定正压生物防护服在高、低风速条件下对病毒气溶胶的防护效率。
噬菌体Phi-X174气溶胶颗粒的质量中值直径约为0.922 µm,背景浓度超过2×10⁴颗粒/立方米。在病毒气溶胶浓度范围为0 - 23 PFU/立方米的不同测试条件下,该防护服对噬菌体Phi-X174气溶胶颗粒的防护效率高于99.9%。气流(P = 0.84)、环境湿度条件(P = 0.33)和采样时间(P = 0.07)对防护效率无统计学影响。
无论气流速率、环境湿度和采样时间如何,正压生物防护服对噬菌体Phi-X174气溶胶均具有较高的防护效率。