LI-COR Biosciences, Lincoln, NE 68504, USA.
Anal Biochem. 2012 Jul 1;426(1):27-9. doi: 10.1016/j.ab.2012.04.004. Epub 2012 Apr 7.
In this study, we applied tyramide signal amplification (TSA) to fluorescence enzyme-linked immunosorbent assay (ELISA) employing horseradish peroxidase (HRP) as the detection enzyme. When used with a human epidermal growth factor ELISA kit, the TSA method led to a >100-fold increase in fluorescence signal intensity in comparison to an unamplified method. It also showed wider dynamic range and better sensitivity compared to a conventional method using tetramethylbenzidine as the HRP substrate.
在这项研究中，我们将辣根过氧化物酶（HRP）作为检测酶应用于荧光酶联免疫吸附测定（ELISA）中的酪胺信号放大（TSA）。当与人类表皮生长因子 ELISA 试剂盒一起使用时，与未放大的方法相比，TSA 方法导致荧光信号强度增加了 100 多倍。与使用四甲基联苯胺作为 HRP 底物的传统方法相比，它还显示出更宽的动态范围和更好的灵敏度。
