利用多粘类芽孢杆菌 PoxB 作为融合伴侣在大肠杆菌中形成活性包涵体。
Active inclusion body formation using Paenibacillus polymyxa PoxB as a fusion partner in Escherichia coli.
机构信息
Systems and Synthetic Biology Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
出版信息
Anal Biochem. 2012 Jul 1;426(1):63-5. doi: 10.1016/j.ab.2012.04.002. Epub 2012 Apr 7.
Overexpression of Paenibacillus polymyxa PoxB in Escherichia coli induced the formation of inclusion bodies. An enzyme assay showed that the inclusion bodies exhibited PoxB activity, indicating that they were biologically active. Fusion of GFP and Bacillus subtilis AmyE to the C-terminus of the PoxB also induced the formation of biologically active aggregates when they were overexpressed in E. coli. Therefore, P. polymyxa PoxB can be used as a fusion partner to promote the formation of active inclusion bodies in E. coli.
多粘类芽孢杆菌 PoxB 在大肠杆菌中的过表达诱导包涵体的形成。酶活性分析表明，这些包涵体具有 PoxB 活性，表明它们具有生物活性。GFP 和枯草芽孢杆菌 AmyE 与 PoxB 的 C 端融合，当它们在大肠杆菌中过表达时，也诱导了生物活性聚集体的形成。因此，多粘类芽孢杆菌 PoxB 可用作融合伴侣，促进大肠杆菌中活性包涵体的形成。
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