[Application of an integrated omics analysis for the discovery of biomarkers for osteosarcoma].

OBJECTIVE

To apply an integrated omics analysis so as to discover potential biomarkers for an early diagnosis of osteosarcoma.

METHODS

Gene chip and surface-enhanced laser desorption/ionization-time of flight-mass spectra (SELDI-TOF-MS) were employed to screen the marker genes and serum markers of osteosarcoma respectively. The associations of potential biomarkers from the SELDI data and microarray analysis were further inferred by Link-test to identify stable and specific diagnostic biomarkers.

RESULTS

A total of 653 pre-biomarkers were found in osteosarcoma cells. And six differentially expressed protein peaks with strong statistical significances were detected by SELDI-TOF-MS in the patient's serum samples. Thirteen potential biomarkers for an early diagnosis of osteosarcoma were screened by Link-test.

CONCLUSION

As an effective method of identifying osteosarcoma biomarkers, the integrated omics analysis may serve as a monitoring platform for an early diagnosis of osteosarcoma.