[泌乳素瘤相关微小RNA的差异表达分析]

[Differential expression analysis of prolactinoma-related microRNAs].

作者信息

Chen Yun-xiang, Li Qiang, Wang Cheng-de, Su Zhi-peng, Li Wei-qiang, Chen Xian-bin, Wu Zhe-bao

机构信息

Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2012 Feb 7;92(5):320-3.

PMID:22490835

Abstract

OBJECTIVE

To explore the relationship between the prolactinoma-related microRNAs (miRNA) and the development, growth and hormone secretion of prolactinoma.

METHODS

The technique of Solexa sequencing was employed to analyze the differential expressions of prolactinoma and normal anterior pituitary gland samples. And the stem-loop real-time polymerase chain reaction (PCR) was utilized for confirmation.

RESULTS

According to the differentially expressed profiles of miRNAs, 4 miRNAs were down-regulated (miR-130a, miR-199b-3p, miR-200b, miR-125b, P < 0.05) and 6 miRNAs up-regulated (miR-342-3p, miR-432, miR-23b, miR-493, miR-493(), miR-664(), P < 0.05). The expression levels of miR-493(*) and miR-432 had a significant positive correlation with the serum level of prolactin (r = 0.47, P < 0.05; r = 0.528, P < 0.01) while miR-342-3p a significantly positive correlation with the invasiveness (r = 0.402, P < 0.05).

CONCLUSION

miRNAs are differentially expressed between normal anterior pituitary gland and prolactinomas, between invasive and localized prolactinomas and among different hormone secretion levels. It suggests that miRNAs may be involved in the physiological process of development, growth and hormone secretion of prolactinoma.

摘要

目的

探讨泌乳素瘤相关微小RNA（miRNA）与泌乳素瘤的发生、生长及激素分泌之间的关系。

方法

采用Solexa测序技术分析泌乳素瘤与正常垂体前叶样本的差异表达情况，并利用茎环实时聚合酶链反应（PCR）进行验证。

结果

根据miRNA的差异表达谱，4种miRNA表达下调（miR-130a、miR-199b-3p、miR-200b、miR-125b，P<0.05），6种miRNA表达上调（miR-342-3p、miR-432、miR-23b、miR-493、miR-493*、miR-664*，P<0.05）。miR-493*和miR-432的表达水平与血清泌乳素水平呈显著正相关（r=0.47，P<0.05；r=0.528，P<0.01），而miR-342-3p与侵袭性呈显著正相关（r=0.402，P<0.05）。