Li Li, Zhou Dilys, Wang Xiu-mei, Wang Xiao-ping, Cui Fu-zhai, Lu Yu-jie, Huang Yi-fei
Department of Ophthalmology, the General Hospital of PLA, Beijing 100853, China.
Zhonghua Yan Ke Za Zhi. 2012 Jan;48(1):20-6.
To investigate the expression of matrix metalloproteinase-2 (MMP-2) and Tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in rabbit corneas implanted with modified titanium skirt of keratoprosthesis in order to explore the potential roles.
A total of 20 New Zealand white rabbits with corneal alkali burn in right eye rabbit corneas were divided into three groups. There were 6 animals in each group. Skirt of hydroxyapatite/Sandblast-Titanium and Sandblast-Titanium were inserted into the corneal stroma of rabbits in group A and group B. The group C did not insert skirt as surgical control.2 rabbits were as normal control D group. A total of 20 New Zealand white rabbits were divided into four groups with the same way. The expression of MMP-2 and TIMP-2 was determined by immunohistochemistry at 1 month, 3 months. The expression of MMP-2 and TIMP-2 mRNA level was determined by real time-polymerase chain reaction, and its protein level was determined by western blot. The optical cylinder was implanted to rabbit corneas, which were implanted with modified titanium skirt after 3 months.
There was one case of corneal dissolution being found in group F. MMP-2 and TIMP-2 immunoreactivities were expressed in the normal corneas, predominantly in the corneal epithelium. After injury, immunoreactivities of both MMP-2 and TIMP-2 were increased notably in the healing corneal epithelium, infiltrating inflammatory cells, stromal fibroblasts and in growing vascular endothelial cells. The expression of MMP-2 was lower in group A and E than that in group B and F after 1 month and 3 months (t = 12.05, 2.93, 12.006, 3.781, P < 0.05). The Western blot revealed no significant differences of MMP-2 mRNA between group 3 months and 2 weeks (t = 2.104, P > 0.05); MMP-2 immunoreactivities were absent or lowly expressed predominantly in the corneal epithelium of normal corneas. The expression of MMP-2, TIMP-2 mRNA level was parallel that of protein level.
The expression of MMP-2 was lower in the corneal tissue sections of HA/SB-Ti skirt inserted eyes than that in the tissue sections of SB-Ti skirt inserted eyes. The studies of MMP-2, TIMP-2 can provide a new way to prevent the incidence of corneal dissolving after surgery for keratoprosthesis.
研究基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶组织抑制剂-2（TIMP-2）在植入改良钛裙人工角膜的兔角膜中的表达情况，以探讨其潜在作用。
选取20只右眼角膜碱烧伤的新西兰白兔，将其分为三组，每组6只。A组和B组分别将羟基磷灰石/喷砂钛裙和喷砂钛裙植入兔角膜基质层，C组不植入裙作为手术对照，另外2只作为正常对照D组。共20只新西兰白兔按同样方法分为四组。在1个月、3个月时通过免疫组织化学法检测MMP-2和TIMP-2的表达。通过实时聚合酶链反应检测MMP-2和TIMP-2 mRNA水平，通过蛋白质印迹法检测其蛋白水平。3个月后给植入改良钛裙的兔角膜植入柱镜。
F组发现1例角膜溶解。MMP-2和TIMP-2免疫反应在正常角膜中表达，主要在角膜上皮。损伤后，MMP-2和TIMP-2在愈合的角膜上皮、浸润的炎性细胞、基质成纤维细胞和新生血管内皮细胞中的免疫反应均显著增加。1个月和3个月后，A组和E组MMP-2的表达低于B组和F组（t = 12.05，2.93，12.006，3.781，P < 0.05）。蛋白质印迹显示3个月组和2周组MMP-2 mRNA无显著差异（t = 2.104，P > 0.05）；MMP-2免疫反应在正常角膜上皮中无或低表达。MMP-2、TIMP-2 mRNA水平与蛋白水平呈平行关系。
植入HA/SB-Ti裙眼的角膜组织切片中MMP-2的表达低于植入SB-Ti裙眼的组织切片。对MMP-2、TIMP-2的研究可为预防人工角膜术后角膜溶解的发生提供新途径。
