Yuan Yuan, Wang Jing-Yao, Yuan Fang, Yu Yong-Hao, Wang Guo-Lin
Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin, China.
Zhonghua Yi Xue Za Zhi. 2012 Feb 21;92(7):435-9. doi: 10.3760/cma.j.issn.00376-2491-2012.07.002.
To investigate the change in glycogen synthase kinase-3β (GSK-3β) in spinal cord neurons in rats with incisional pain (IP)-remifentanil-induced hyperalgesia.
32 SD male rats (240 - 260 g) were randomly divided into 4 groups (n = 8 each): group R, group I, group R + I and group C. IP was established as Brennan's description. Paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were measured 24h before anesthesia and 2, 6, 24, 48 h after anesthesia. The rats were sacrificed after the last threshold measurement. The expressions of GSK-3β mRNA in rats' spinal cord neurons were determined by real-time PCR. The expressions of GSK-3β and pGSK-3β in rats' spinal cord neurons were determined by Western blot.
Remifentanil-induced hyperalgesia developed in group R, I and R + I. The expression of GSK-3β mRNA and the expression of GSK-3β in rats' spinal cord neurons were highest in group R + I. In addition, the ratio of pGSK-3β/GSK-3β was smallest in group R + I.
These data indicate that the increased GSK-3β activity in rats spinal cord neurons is involved in remifentanil-induced hyperalgesia.
探讨切口痛（IP）-瑞芬太尼诱导的痛觉过敏大鼠脊髓神经元中糖原合酶激酶-3β（GSK-3β）的变化。
将32只SD雄性大鼠（240 - 260 g）随机分为4组（每组n = 8）：R组、I组、R + I组和C组。按照Brennan的描述建立IP模型。在麻醉前24小时以及麻醉后2、6、24、48小时测量 paw withdrawal threshold（PWT）和 paw withdrawal latency（PWL）。在最后一次阈值测量后处死大鼠。通过实时PCR测定大鼠脊髓神经元中GSK-3β mRNA的表达。通过蛋白质免疫印迹法测定大鼠脊髓神经元中GSK-3β和pGSK-3β的表达。
R组、I组和R + I组出现瑞芬太尼诱导的痛觉过敏。大鼠脊髓神经元中GSK-3β mRNA的表达以及GSK-3β的表达在R + I组中最高。此外，pGSK-3β/GSK-3β的比值在R + I组中最小。
这些数据表明大鼠脊髓神经元中GSK-3β活性增加与瑞芬太尼诱导的痛觉过敏有关。
