Zhou X, Wang L
School of Pharmacy, Shandong University, Ji'nan, Shandong, China.
Drug Discov Ther. 2010 Apr;4(2):62-9.
Single-particle tracking (SPT) techniques have been developing rapidly in the field of cellular biology as a means of unraveling the diffusion dynamics of bio-molecules and the function of proteins in the regulation of cellular activity at single molecule sensitivity and nanometer spatial resolution. Suitable probes and technological improvements have made SPT more accessible than it used to be and paved the way for broad applications in cellular biology. This review summarizes the principles of SPT and discusses the main findings yielded by the technique and its contribution to the understanding of proteins in living cells.
单粒子追踪（SPT）技术在细胞生物学领域发展迅速，它能够以单分子灵敏度和纳米空间分辨率揭示生物分子的扩散动力学以及蛋白质在细胞活动调控中的功能。合适的探针和技术改进使SPT比以往更容易实现，并为其在细胞生物学中的广泛应用铺平了道路。本文综述了SPT的原理，讨论了该技术产生的主要发现及其对理解活细胞中蛋白质的贡献。
