Emam A, Eweis M, Elbadry M
Department of Biochemistry, Faculty of Agriculture, Fayoum University, Fayoum, Egypt.
Drug Discov Ther. 2010 Dec;4(6):399-404.
Bioassay-guided separation with an eye toward antifungal activity led to the isolation of the new alkaloid 5-(1̀,1̀-dimethylallyl)-8-hydroxyfuro[2-3-b] quinoline (1) and the known biscoumarin daphnoretin (2) as the active constituents of the chloroform extract obtained from the leaves of Ruta chalepensis. The structures of the metabolites were elucidated on the basis of their spectral characteristics (NMR, UV, and MS) and were compared with the literature. The antifungal activity of the isolated compounds was evaluated against the phytopathogenic fungi Rhizoctonia solani, Sclerotium rolfsii, and Fusarium solani, which cause root-rot and wilt diseases in several economically important food crops such as potato, sugar beet, and tomato.
以抗真菌活性为导向的生物测定引导分离，从白藓叶的氯仿提取物中分离出了新生物碱5-(1̀,1̀-二甲基烯丙基)-8-羟基呋喃并[2-3-b]喹啉(1)和已知双香豆素瑞香素(2)作为活性成分。根据代谢产物的光谱特征(NMR、UV和MS)阐明了其结构，并与文献进行了比较。评估了分离出的化合物对植物病原真菌立枯丝核菌、齐整小核菌和茄类镰刀菌的抗真菌活性，这些真菌在马铃薯、甜菜和番茄等几种经济上重要的粮食作物中引起根腐病和枯萎病。
