Salib Josline Y, El-Toumy Sayed A, Hassan Emad M, Shafik Nabila H, Abdel-Latif Sally M, Brouard Ignacio
a Chemistry of Tannins Department , National Research Centre , Dokki, Cairo , Egypt.
Nat Prod Res. 2014;28(17):1335-42. doi: 10.1080/14786419.2014.903395. Epub 2014 Apr 4.
Bioassay-guided isolation of methanol extract of Ruta graveolens L. leaves yielded a new quinoline alkaloid, (4S) 1,4-dihydro-4-methoxy-1,4-dimethyl-3-(3-methylbut-2-enyl)quinoline 2,7-diol, and nine phenolic compounds including rutin as a major compound. Structures of the isolated compounds were determined by using chromatography, UV, HR-ESI-MS and 1D/2D (1)H/(13)C NMR spectroscopy. The uterotonic activity of methanol extract fractions (ethyl acetate, n-butanol and aqueous fraction) as well as the isolated major compounds was tested in the isolated mouse uterus in vitro. The n-butanol-soluble fraction was found to demonstrate the most potent uterotonic activity in a dose-dependent manner, also the major isolated compound rutin revealed the occurrence of an uterotonic response, which was maximum at a concentration level of 0.25 mg/mL, accounting for 68.7% of that exhibited by the chosen concentration of oxytocin.
对芸香叶甲醇提取物进行生物测定导向分离，得到一种新的喹啉生物碱，即(4S)1,4 - 二氢 - 4 - 甲氧基 - 1,4 - 二甲基 - 3 - (3 - 甲基丁 - 2 - 烯基)喹啉 - 2,7 - 二醇，以及九种酚类化合物，其中芦丁为主要化合物。通过色谱法、紫外光谱、高分辨电喷雾电离质谱和一维/二维氢谱/碳谱核磁共振光谱确定了分离得到的化合物的结构。在离体小鼠子宫中体外测试了甲醇提取物各馏分（乙酸乙酯、正丁醇和水馏分）以及分离得到的主要化合物的子宫收缩活性。发现正丁醇可溶馏分以剂量依赖方式表现出最强的子宫收缩活性，同样，分离得到的主要化合物芦丁也显示出子宫收缩反应，在浓度为0.25mg/mL时达到最大值，占所选浓度催产素所表现活性的68.7%。
