采用反向遗传学方法研究猫传染性腹膜炎。
A reverse genetics approach to study feline infectious peritonitis.
机构信息
Institut für Virologie, Justus-Liebig-Universität Giessen, Giessen, Germany.
出版信息
J Virol. 2012 Jun;86(12):6994-8. doi: 10.1128/JVI.00023-12. Epub 2012 Apr 4.
Abstract
Feline infectious peritonitis (FIP) is a lethal immunopathological disease caused by feline coronaviruses (FCoVs). Here, we describe a reverse genetics approach to study FIP by assessing the pathogenicity of recombinant type I and type II and chimeric type I/type II FCoVs. All recombinant FCoVs established productive infection in cats, and recombinant type II FCoV (strain 79-1146) induced FIP. Virus sequence analyses from FIP-diseased cats revealed that the 3c gene stop codon of strain 79-1146 has changed to restore a full-length open reading frame (ORF).
摘要
猫传染性腹膜炎（FIP）是由猫冠状病毒（FCoV）引起的致命免疫病理疾病。在这里，我们描述了一种通过评估重组 I 型和 II 型以及嵌合 I/II 型 FCoV 的致病性来研究 FIP 的反向遗传学方法。所有重组 FCoV 均可在猫中建立有效的感染，重组 II 型 FCoV（株 79-1146）可诱导 FIP。从患有 FIP 的猫中分离的病毒序列分析表明，株 79-1146 的 3c 基因终止密码子已发生改变，以恢复全长开放阅读框（ORF）。
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