Plant Cell Biotechnology Department, Central Food Technological Research Institute-Laboratory of the Council of Scientific and Industrial Research-New Delhi, Mysore 570020, India.
Plant Foods Hum Nutr. 2012 Jun;67(2):162-70. doi: 10.1007/s11130-012-0285-1.
Folate (vitamin B₉) content was evaluated in 10 varieties of coriander with the aim of enhancing its concentration and stability, because of three reasons: 1) coriander is among a few widely used greens in the world and suits many cuisines, 2) folate deficiency is prevalent in developing countries causing anaemia, infant mortality and neural tube closure defects, and 3) natural folate is preferred due to doubts about health risks associated with the synthetic form. In C. sativum, the highest folate content of 1,577 μg/100 g DW was found in var. GS4 Multicut foliage of mature plants (marketable stage) with an insignificantly higher content (1,599.74 μg/100 g DW) at flowering, which is a stage not preferred in markets. In callus cultures treated with plant growth regulators (GRs) (6-benzylaminopurine, kinetin and abscisic acid) substantial increase in folate occurred after 6 h, whereas elicitors (methyl jasmonate and salicylic acid) caused rapid 2-fold increase of folate, particularly in response to salicylic acid. Based on these observations, foliar applications were done for in vivo plants, where salicylic acid (250 μM, 24 h) also enhanced folate level by 2-folds (3,112.33 μg/100 g DW), although the content varied with diurnal rhythms. Stability of folates in treated coriander foliage was 10 % higher than in untreated foliage when stored at 25 °C and 4 °C. This study has established for the first time that coriander foliage is rich in folates, which can be doubled by elicitation and impart 10 % more stability than control during processing and storage.
评估了 10 种芫荽品种中的叶酸（维生素 B₉）含量，目的是提高其浓度和稳定性，原因有三：1）芫荽是世界上少数几种广泛使用的绿色蔬菜之一，适合许多菜肴；2）叶酸缺乏在发展中国家很普遍，会导致贫血、婴儿死亡率和神经管闭合缺陷；3）由于对合成叶酸的健康风险存在疑虑，因此更喜欢天然叶酸。在芫荽属植物中，成熟植株（可销售阶段）的 GS4 Multicut 叶片中的叶酸含量最高，为 1577μg/100g DW，开花期的叶酸含量略高（1599.74μg/100g DW），但在市场上并不受欢迎。用植物生长调节剂（GRs）（6-苄基氨基嘌呤、激动素和脱落酸）处理愈伤组织后，6 小时后叶酸含量显著增加，而诱导剂（茉莉酸甲酯和水杨酸）使叶酸迅速增加 2 倍，尤其是对水杨酸的反应。基于这些观察结果，对活体植物进行了叶面喷施，其中水杨酸（250μM，24 小时）也使叶酸水平增加了 2 倍（3112.33μg/100g DW），尽管含量随昼夜节律而变化。在 25°C 和 4°C 下储存时，处理过的芫荽叶片中的叶酸稳定性比未处理的叶片高 10%。本研究首次证实芫荽叶片富含叶酸，用诱导剂处理可使叶酸含量增加一倍，在加工和储存过程中的稳定性比对照提高 10%。
