The First Department of General Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, China.
Med Oncol. 2012 Dec;29(5):3108-12. doi: 10.1007/s12032-012-0233-9. Epub 2012 Apr 11.
MicroRNAs (miRNAs) are non-coding RNAs that have been shown to be aberrantly expressed in several tumor types. Of these miRNAs, miR-22 as tumor suppressor has been shown to play a crucial role in human carcinogenesis. However, its association with the clinicopathological features of colorectal cancer has yet to be addressed. In this study, we compared the expression of miR-22 between colorectal cancer tissues and the normal adjacent mucosa using a quantitative real-time RT-PCR. The association of miR-22 expression with clinicopathological characters was analyzed by appropriate statistical analysis. Kaplan-Meier analysis and Cox proportional hazards regression models were used to investigate the associations of miR-22 expression with survival of patients. Results showed that the relative expression levels of miR-22 were significantly lower in colorectal cancer tissues than those in the normal adjacent mucosa, and low expression of miR-22 correlated with liver metastasis. Kaplan-Meier analysis indicated that patients with reduced miR-22 had a poor overall survival. Moreover, the multivariate analysis showed that reduced expression of miR-22 was an independent predictor of overall survival. Our data indicate the potential of miR-22 as a novel prognostic biomarker for CRC.
MicroRNAs (miRNAs) 是一类非编码 RNA，已被证实存在于多种肿瘤类型中异常表达。在这些 miRNA 中，miR-22 作为肿瘤抑制因子，在人类肿瘤发生中发挥着至关重要的作用。然而，miR-22 与结直肠癌的临床病理特征之间的关联尚未得到解决。在本研究中，我们使用定量实时 RT-PCR 比较了结直肠癌组织和正常相邻黏膜中 miR-22 的表达。通过适当的统计分析，分析了 miR-22 表达与临床病理特征的关系。Kaplan-Meier 分析和 Cox 比例风险回归模型用于研究 miR-22 表达与患者生存的关系。结果表明，miR-22 的相对表达水平在结直肠癌组织中明显低于正常相邻黏膜，并且 miR-22 的低表达与肝转移相关。Kaplan-Meier 分析表明，miR-22 表达降低的患者总生存较差。此外，多变量分析表明，miR-22 表达降低是总生存的独立预测因子。我们的数据表明，miR-22 可能成为 CRC 的一种新的预后生物标志物。
