Barbolov Momchil, Slavova Svetla, Nedeva Neda, Ivanov Krasimir, Kolev Nikola, Komosinska-Vassev Katarzyna, Ivanova Diana, Vankova Deyana, Kiselova-Kaneva Yoana
Department of Biochemistry, Molecular Medicine and Nutrigenomics, Faculty of Pharmacy, Medical University "Prof. Dr. Paraskev Stoyanov", 9000 Varna, Bulgaria.
Department of Biology, Faculty of Pharmacy, Medical University "Prof. Dr. Paraskev Stoyanov", 9000 Varna, Bulgaria.
Int J Mol Sci. 2025 Jul 25;26(15):7196. doi: 10.3390/ijms26157196.
Dysregulation in miRNA expression has been reported in a variety of tumors, including colorectal cancer (CRC), where adiponectin regulates a number of processes related to tumorigenesis. The aim of this study was to identify a panel of heavily and consistently altered miRNAs in CRC that affect adiponectin signaling based on bioinformatics analysis and cross-referencing the available literature. Bioinformatics tools were used to analyze publicly available datasets to identify miRNAs targeting the adiponectin pathway that are substantially dysregulated in CRC. In parallel, a comprehensive literature review was conducted to gather and explore existing knowledge on the relationship between CRC, adiponectin signaling, and miRNA dysregulation. Bioinformatics analysis revealed a set of miRNAs that target adiponectin signaling and are consistently altered in CRC. Several candidate miRNAs, including miR-215-5p, miR-340-5p, miR-181a-5p, miR-150-5p, miR-96-5p, miR-19a-3p, and miR-21-5p, were identified as potential key regulators of the adiponectin cascade, while also being systemically dysregulated in CRC. Through gene ontology enrichment analysis, we further elucidated the biological processes and pathways impacted by these miRNAs, providing insight into their contributions to CRC. The literature review did not identify any previously reported shared connection between these miRNAs, adiponectin signaling, and CRC pathogenesis.
据报道，包括结直肠癌（CRC）在内的多种肿瘤中都存在miRNA表达失调的情况，其中脂联素调节许多与肿瘤发生相关的过程。本研究的目的是基于生物信息学分析并参考现有文献，确定一组在CRC中大量且持续改变的miRNA，这些miRNA会影响脂联素信号传导。使用生物信息学工具分析公开可用的数据集，以识别在CRC中显著失调的靶向脂联素途径的miRNA。同时，进行了全面的文献综述，以收集和探索关于CRC、脂联素信号传导和miRNA失调之间关系的现有知识。生物信息学分析揭示了一组靶向脂联素信号传导且在CRC中持续改变的miRNA。包括miR-215-5p、miR-340-5p、miR-181a-5p、miR-150-5p、miR-96-5p、miR-19a-3p和miR-21-5p在内的几种候选miRNA被确定为脂联素级联反应的潜在关键调节因子，同时在CRC中也存在系统性失调。通过基因本体富集分析，我们进一步阐明了这些miRNA影响的生物学过程和途径，深入了解了它们对CRC的作用。文献综述未发现这些miRNA、脂联素信号传导和CRC发病机制之间有任何先前报道的共同联系。
