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用于高空间分辨率湿地土壤微生物群落结构和功能特征描述的冷冻取芯方法。

Freeze-coring method for characterization of microbial community structure and function in wetland soils at high spatial resolution.

机构信息

Institute of Biogeochemistry and Pollutant Dynamics, ETH Zurich, Zurich, Switzerland.

出版信息

Appl Environ Microbiol. 2012 Jun;78(12):4501-4. doi: 10.1128/AEM.00133-12. Epub 2012 Apr 6.

DOI:10.1128/AEM.00133-12
PMID:22492456
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3370540/
Abstract

A simple freeze-coring method was developed to obtain structurally intact cores from wetland soils. A copper tube was inserted into the wetland and filled with ethanol and dry ice to freeze the surrounding soil. Biological structure and function could be analyzed, and labile compounds such as mRNA were recovered.

摘要

一种简单的冷冻取芯方法被开发出来，用于从湿地土壤中获得结构完整的岩芯。将一根铜管插入湿地，并用乙醇和干冰填充，以冷冻周围的土壤。可以对生物结构和功能进行分析，并回收易变的化合物，如 mRNA。

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