George E. Wahlen Department of Veterans Affairs Medical Center, Salt Lake City, Utah, USA.
J Biomed Mater Res A. 2012 Jul;100(7):1888-900. doi: 10.1002/jbm.a.34123. Epub 2012 Apr 10.
Currently, the majority of animal models that are used to study biofilm-related infections use planktonic bacterial cells as initial inocula to produce positive signals of infection in biomaterials studies. However, the use of planktonic cells has potentially led to inconsistent results in infection outcomes. In this study, well-established biofilms of methicillin-resistant Staphylococcus aureus were grown and used as initial inocula in an animal model of a Type IIIB open fracture. The goal of the work was to establish, for the first time, a repeatable model of biofilm implant-related osteomyelitis, wherein biofilms were used as initial inocula to test combination biomaterials. Results showed that 100% of animals that were treated with biofilms developed osteomyelitis, whereas 0% of animals not treated with biofilm developed infection. The development of this experimental model may lead to an important shift in biofilm and biomaterials research by showing that when biofilms are used as initial inocula, they may provide additional insights into how biofilm-related infections in the clinic develop and how they can be treated with combination biomaterials to eradicate and/or prevent biofilm formation.
目前，用于研究生物膜相关感染的大多数动物模型都使用浮游细菌细胞作为初始接种物，以在生物材料研究中产生感染的阳性信号。然而，浮游细胞的使用可能导致感染结果不一致。在这项研究中，我们建立了成熟的耐甲氧西林金黄色葡萄球菌生物膜，并将其用作 IIIB 型开放性骨折动物模型中的初始接种物。这项工作的目的是首次建立可重复的生物膜植入物相关骨髓炎模型，其中生物膜被用作初始接种物来测试组合生物材料。结果表明，用生物膜治疗的动物 100%发生骨髓炎，而未用生物膜治疗的动物 0%发生感染。该实验模型的建立可能会推动生物膜和生物材料研究的重要转变，表明当生物膜作为初始接种物时，它们可能会提供更多关于临床中生物膜相关感染如何发展以及如何用组合生物材料来消除和/或预防生物膜形成来治疗这些感染的见解。
