Department of Bioengineering, University of Utah, Salt Lake City, UT, USA.
Curr Microbiol. 2011 Jun;62(6):1657-63. doi: 10.1007/s00284-011-9908-2. Epub 2011 Mar 25.
Biofilm-related infections have become a major clinical concern. Typically, animal models that involve inoculation with planktonic bacteria have been used to create positive infection signals and examine antimicrobial strategies for eradicating or preventing biofilm-related infection. However, it is estimated that 99.9% of bacteria in nature dwell in established biofilms. As such, open wounds have significant potential to become contaminated with bacteria that reside in a well-established biofilm. In this study, a modified CDC biofilm reactor was developed to repeatably grow mature biofilms of Staphylococcus aureus on the surface of polyetheretherketone (PEEK) membranes for inoculation in a future animal model of orthopaedic implant biofilm-related infection. Results indicated that uniform, mature biofilms repeatably grew on the surface of the PEEK membranes.
生物膜相关感染已成为一个主要的临床关注点。通常，使用涉及浮游细菌接种的动物模型来产生阳性感染信号，并研究消除或预防生物膜相关感染的抗菌策略。然而，据估计，自然界中 99.9%的细菌都存在于已建立的生物膜中。因此，开放性伤口有很大的可能被存在于成熟生物膜中的细菌污染。在这项研究中，开发了一种改良的 CDC 生物膜反应器，以便在未来的骨科植入物生物膜相关感染动物模型中重复接种，在聚醚醚酮（PEEK）膜表面上重复形成成熟的金黄色葡萄球菌生物膜。结果表明，均匀、成熟的生物膜可在 PEEK 膜表面上重复形成。
