Department of Textile Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
J Biomed Mater Res A. 2012 Jul;100(7):1907-18. doi: 10.1002/jbm.a.34131. Epub 2012 Apr 10.
A critical element in tissue engineering involves the fabrication of a three-dimensional scaffold. The scaffold provides a space for new tissue formation, supports cellular ingrowth, and proliferation and mimics many roles of the extracellular matrix. Poly(3-hydroxybutyrate) (PHB) is the most thoroughly investigated member of the polyhydroxyalkanoates (PHAs) family that has various degrees of biocompatibility and biodegradability for tissue engineering applications. In this study, we fabricated PHB scaffolds by utilizing electrospinning and salt-leaching procedures. The behavior of monkey epithelial kidney cells (Vero) and mouse mesenchymal stem cells (mMSCs) on these scaffolds was compared by the MTS assay and scanning electron microscopy. Additionally, this study investigated the mechanical and physical properties of these scaffolds by measuring tensile strength and modulus, dynamic contact angle and porosity. According to our results, the salt-leached scaffolds showed more wettability and permeability, but inferior mechanical properties when compared with nanofibrous scaffolds. In terms of cell response, salt-leached scaffolds showed enhanced Vero cell proliferation, whereas both scaffolds responded similarly in the case of mMSCs proliferation. In brief, nanofibrous scaffolds can be a better substrate for cell attachment and morphology.
在组织工程中，一个关键的元素涉及到三维支架的制造。支架提供了新组织形成的空间，支持细胞的向内生长、增殖，并模拟细胞外基质的许多功能。聚（3-羟基丁酸酯）（PHB）是聚羟基烷酸酯（PHA）家族中研究最透彻的成员，具有不同程度的生物相容性和可生物降解性，适用于组织工程应用。在这项研究中，我们通过静电纺丝和盐析法制备了 PHB 支架。通过 MTS 测定法和扫描电子显微镜比较了这些支架上猴肾上皮细胞（Vero）和小鼠间充质干细胞（mMSCs）的行为。此外，通过测量拉伸强度和模量、动态接触角和孔隙率，研究了这些支架的机械和物理性能。根据我们的结果，与纳米纤维支架相比，盐析支架具有更好的润湿性和渗透性，但机械性能较差。就细胞反应而言，盐析支架显示出增强的 Vero 细胞增殖，而在 mMSCs 增殖的情况下，两种支架的反应相似。简而言之，纳米纤维支架可以作为细胞附着和形态的更好基质。
