Karbasi Saeed, Fekrat Farnoosh, Semnani Daryoush, Razavi Shahnaz, Zargar Elham Naghash
Department of Biomaterials and Tissue Engineering, School of Advance Technology in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Textile Engineering, Isfahan University of Technology, Isfahan, Iran.
Adv Biomed Res. 2016 Nov 28;5:180. doi: 10.4103/2277-9175.194802. eCollection 2016.
One of the new methods of scaffold fabrication is a nano-micro hybrid structure in which the properties of the scaffold are improved by introducing nanometer and micrometer structures. This method could be suitable for scaffold designing if some features improve.
In this study, electrospun nanofibers of 9% weight solution of poly (3-hydroxybutyrate) (P3HB) and a 15% weight of chitosan by trifluoroacetic acid were coated on both the surface of a silk knitted substrate in the optimum condition to improve the mechanical properties of scaffolds for cartilage tissue engineering application. These hybrid nano-micro fibrous scaffolds were characterized by structural and mechanical evaluation methods.
Scanning electron microscopy values and porosity analysis showed that average diameter of nanofibers was 584.94 nm in electrospinning part and general porosity was more than 80%. Fourier transform infrared spectroscopy results indicated the presence of all elements without pollution. The tensile test also stated that by electrospinning, as well as adding chitosan, both maximum strength and maximum elongation increased to 187 N and 10 mm. It means that the microfibrous part of scaffold could affect mechanical properties of nano part of the hybrid scaffold, significantly.
It could be concluded that P3HB-chitosan/silk hybrid scaffolds can be a good candidate for cartilage tissue engineering.
支架制造的新方法之一是纳米-微米混合结构，其中通过引入纳米和微米结构来改善支架的性能。如果某些特性得到改善，这种方法可能适用于支架设计。
在本研究中，将9%重量的聚（3-羟基丁酸酯）（P3HB）电纺纳米纤维和15%重量的壳聚糖通过三氟乙酸在最佳条件下涂覆在丝织基底的表面，以改善用于软骨组织工程应用的支架的机械性能。这些混合纳米-微米纤维支架通过结构和力学评估方法进行表征。
扫描电子显微镜值和孔隙率分析表明，电纺部分纳米纤维的平均直径为584.94 nm，总孔隙率超过80%。傅里叶变换红外光谱结果表明所有元素均存在且无污染。拉伸试验还表明，通过电纺以及添加壳聚糖，最大强度和最大伸长率均增加到187 N和10 mm。这意味着支架的微纤维部分可显著影响混合支架纳米部分的机械性能。
可以得出结论，P3HB-壳聚糖/丝混合支架可能是软骨组织工程的良好候选材料。
