VU Institute of Biochemistry, Mokslininku 12, Vilnius, Lithuania.
J Virol. 2012 May;86(9):5406. doi: 10.1128/JVI.00347-12.
Despite the fact that multidrug-resistant Klebsiella sp. strains emerge rapidly (Xu J, et al., Adv. Mater. Res. 268-270:1954-1956, 2011) and bacteriophages have been reported to be useful in controlling these bacteria (Kumari S, Harjai K, Chhibber S, J. Med. Microbiol. 60:205-210, 2011), the complete genome sequences of only five Klebsiella phages (four siphoviruses and one myovirus) can be found in databases. In this paper, we report on the complete genome sequence of Klebsiella sp.-infecting bacteriophage vB_KleM_RaK2. With a genome size of 345,809 bp, this is the second largest myovirus and the largest Klebsiella phage sequenced to date. This phage differs substantially from other myoviruses since 411 out of 534 vB_KleM_RaK2 open reading frames have no known functions and lack any reliable database matches. Comparative analysis of the genome sequence of vB_KleM_RaK2 suggests that this phage forms a distinct phylogenetic branch within the family Myoviridae of tailed bacteriophages.
尽管多药耐药的克雷伯氏菌属菌株迅速出现（Xu J，等人，Adv。Mater。Res. 268-270：1954-1956，2011），并且已经报道噬菌体在控制这些细菌方面很有用（Kumari S，Harjai K，Chhibber S，J. Med。 Microbiol. 60：205-210，2011），但数据库中只能找到五种克雷伯氏菌噬菌体（四种丝状噬菌体和一种肌病毒）的完整基因组序列。在本文中，我们报告了感染克雷伯氏菌的噬菌体 vB_KleM_RaK2 的完整基因组序列。该基因组大小为 345809 bp，是第二大亚病毒，也是迄今为止测序的最大克雷伯氏噬菌体。该噬菌体与其他肌病毒有很大不同，因为 vB_KleM_RaK2 的 534 个开放阅读框中有 411 个没有已知功能，并且缺乏任何可靠的数据库匹配。对 vB_KleM_RaK2 基因组序列的比较分析表明，该噬菌体在有尾噬菌体的肌病毒科内形成了一个独特的进化分支。
