Eden C S, Eriksson B, Hanson L A
Infect Immun. 1977 Dec;18(3):767-74. doi: 10.1128/iai.18.3.767-774.1977.
Optimal conditions for in vitro adherence of Escherichia coli to uroepithelial cells, previously shown to more efficient for strains causing acute symptomatic than that for strains causing "asymptomatic" urinary tract infections, were investigated. Uroepithelial cells from fresh morning urine of healthy individuals and E. coli bacteria from patients with various forms of urinary tract infeciton were used. Adhesion was found to vary, between individuals and epithelial cell types, with epithelial cell viability, bacterial cultivation medium and growth phase, number of bacteria added to the epithelial cells, and incubation time and temperature. Adhesion was also influenced by variations in pH and osmolarity. Optimal test conditions were obtained with post-log-phase bacterial cultures grown on nutrient broth when 10(8) bacteria were added to 10(5) epithelial cells and incubated for 60 min. Considerable variation was found between experiments done on different days, whereas the variation between duplicates was small. The method described may provide a useful tool in the study of the host-parasite relationship in urinary tract infections.
此前研究表明，导致急性症状性尿路感染的大肠杆菌菌株比导致“无症状”尿路感染的菌株在体外对尿道上皮细胞的黏附更有效，本文对其最佳条件进行了研究。使用了来自健康个体清晨新鲜尿液的尿道上皮细胞以及来自各种形式尿路感染患者的大肠杆菌。结果发现，黏附情况在个体之间、上皮细胞类型之间存在差异，并且受到上皮细胞活力、细菌培养基和生长阶段、添加到上皮细胞的细菌数量、孵育时间和温度的影响。pH值和渗透压的变化也会影响黏附。当在营养肉汤中生长的对数后期细菌培养物中加入10⁸个细菌并与10⁵个上皮细胞一起孵育60分钟时，可获得最佳测试条件。在不同日期进行的实验之间发现了相当大的差异，而重复实验之间的差异较小。所描述的方法可能为研究尿路感染中的宿主-寄生虫关系提供一个有用的工具。
