应用流式细胞术快速检测碎牛肉中前 6 种非 O157 产志贺毒素大肠埃希氏菌 O 群。
Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry.
机构信息
Escherichia coli Reference Center, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania, USA.
出版信息
J Clin Microbiol. 2012 Jun;50(6):2137-9. doi: 10.1128/JCM.00137-12. Epub 2012 Apr 4.
Abstract
Rapid, sensitive, and highly specific flow-cytometric assays were developed for the detection of the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) O groups in ground beef. The analytical sensitivity of the assays was 2 × 10(3) target cells in a bacterial mixture of 10(5) CFU/ml, and the limit of detection in ground beef was 1 to 10 CFU following 8 h of enrichment. The assays may be utilized for rapid detection of STEC O groups in meat.
摘要
快速、灵敏且高度特异的流式细胞术检测方法被开发出来，用于检测牛肉中排名前六的非 O157 产志贺毒素大肠杆菌（STEC）O 群。该检测方法在细菌混合物中检测 10(5)CFU/ml 的目标细胞时，分析灵敏度为 2×10(3)个细胞，在牛肉中经过 8 小时的富集后，检测限为 1 至 10 CFU。这些检测方法可用于快速检测肉类中的 STEC O 群。
相似文献
1
Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry.应用流式细胞术快速检测碎牛肉中前 6 种非 O157 产志贺毒素大肠埃希氏菌 O 群。
J Clin Microbiol. 2012 Jun;50(6):2137-9. doi: 10.1128/JCM.00137-12. Epub 2012 Apr 4.
2
Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beef.应用多重实时聚合酶链反应检测和分离在-ground 牛肉中的产志贺毒素大肠杆菌血清型 O26、O45、O103、O111、O121 和 O145。
Foodborne Pathog Dis. 2011 May;8(5):601-7. doi: 10.1089/fpd.2010.0773. Epub 2011 Jan 9.
3
Rapid and specific detection of escherichia coli serogroups O26, O45, O103, O111, O121, O145, and O157 in ground beef, beef trim, and produce by loop-mediated isothermal amplification.利用环介导等温扩增技术快速特异性检测碎牛肉、牛肉切块和农产品中的大肠杆菌 O26、O45、O103、O111、O121、O145 和 O157 血清群。
Appl Environ Microbiol. 2012 Apr;78(8):2727-36. doi: 10.1128/AEM.07975-11. Epub 2012 Feb 10.
4
Detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups by ELISA.酶联免疫吸附试验检测六种非 O157 产志贺毒素大肠杆菌 O 群。
Foodborne Pathog Dis. 2012 Nov;9(11):1044-8. doi: 10.1089/fpd.2012.1231.
5
Modification and Matrix Extension of the Bio-Rad iQ-Check E. coli O157:H7, STEC VirX, and STEC SerO Test Kits for the Detection of Shiga Toxin-Producing Escherichia coli (STEC) and Escherichia coli O157 From a Single Enrichment.对 Bio-Rad iQ-Check E. coli O157:H7、STEC VirX 和 STEC SerO 检测试剂盒进行修饰和基质扩展，用于从单一增菌液中检测产志贺毒素大肠埃希氏菌（STEC）和大肠埃希氏菌 O157。
J AOAC Int. 2020 Jan 1;103(1):161-175. doi: 10.5740/jaoacint.19-0254.
6
Rapid O serogroup identification of the six clinically relevant Shiga toxin-producing Escherichia coli by antibody microarray.通过抗体微阵列快速鉴定六种临床相关产志贺毒素大肠杆菌血清群。
J Microbiol Methods. 2013 Jun;93(3):273-6. doi: 10.1016/j.mimet.2013.03.024. Epub 2013 Apr 6.
7
Presence of Shiga toxin-producing Escherichia coli O-groups in small and very-small beef-processing plants and resulting ground beef detected by a multiplex polymerase chain reaction assay.采用多重聚合酶链反应检测小型和超小型牛肉加工场中存在的产志贺毒素大肠杆菌 O 群及其所致的绞碎牛肉。
Foodborne Pathog Dis. 2013 Sep;10(9):789-95. doi: 10.1089/fpd.2012.1445. Epub 2013 Jun 6.
8
Detection of Shiga toxin-producing Escherichia coli (STEC) in ground beef and bean sprouts: Evaluation of culture enrichment conditions.检测 Ground beef 和豆芽中的产志贺毒素大肠杆菌（STEC）：培养富集条件的评估。
Food Res Int. 2018 Jan;103:398-405. doi: 10.1016/j.foodres.2017.10.059. Epub 2017 Oct 31.
9
Further development of sample preparation and detection methods for O157 and the top 6 non-O157 STEC serogroups in cattle feces.牛粪便中O157及6种主要非O157 STEC血清群样本制备和检测方法的进一步发展
J Microbiol Methods. 2014 Oct;105:22-30. doi: 10.1016/j.mimet.2014.06.020. Epub 2014 Jul 12.
10
Development and validation of two SYBR green PCR assays and a multiplex real-time PCR for the detection of Shiga toxin-producing Escherichia coli in meat.两种SYBR Green PCR检测方法及一种多重实时PCR检测方法的开发与验证，用于检测肉类中产生志贺毒素的大肠杆菌。
J Microbiol Methods. 2015 Dec;119:10-7. doi: 10.1016/j.mimet.2015.09.013. Epub 2015 Sep 26.
引用本文的文献
1
The "Big Six": Hidden Emerging Foodborne Bacterial Pathogens.“六大”：隐匿的新兴食源细菌性病原菌
Trop Med Infect Dis. 2022 Nov 7;7(11):356. doi: 10.3390/tropicalmed7110356.
2
A recombinant O-polysaccharide-protein conjugate approach to develop highly specific monoclonal antibodies to Shiga toxin-producing Escherichia coli O157 and O145 serogroups.一种用于开发针对产志贺毒素大肠杆菌O157和O145血清群的高特异性单克隆抗体的重组O-多糖-蛋白质偶联方法。
PLoS One. 2017 Oct 5;12(10):e0182452. doi: 10.1371/journal.pone.0182452. eCollection 2017.
3
A review on detection methods used for foodborne pathogens.一份关于用于食源性病原体的检测方法的综述。
Indian J Med Res. 2016 Sep;144(3):327-338. doi: 10.4103/0971-5916.198677.
4
Comparison of O-Antigen Gene Clusters of All O-Serogroups of Escherichia coli and Proposal for Adopting a New Nomenclature for O-Typing.大肠杆菌所有O血清群的O抗原基因簇比较及O分型新命名法的采用建议
PLoS One. 2016 Jan 29;11(1):e0147434. doi: 10.1371/journal.pone.0147434. eCollection 2016.
本文引用的文献
1
Detection of O antigens in Escherichia coli.检测大肠杆菌中的 O 抗原。
Anim Health Res Rev. 2011 Dec;12(2):169-85. doi: 10.1017/S1466252311000193.
2
Detection of Shiga toxin-producing Escherichia coli O26, O45, O103, O111, O113, O121, O145, and O157 serogroups by multiplex polymerase chain reaction of the wzx gene of the O-antigen gene cluster.采用 O 抗原基因簇 wzx 基因的多重聚合酶链反应检测产志贺毒素大肠杆菌 O26、O45、O103、O111、O113、O121、O145 和 O157 血清群。
Foodborne Pathog Dis. 2011 May;8(5):651-2. doi: 10.1089/fpd.2010.0769.
3
Multiplex PCR detection of Shiga toxin-producing Escherichia coli strains belonging to serogroups O157, O103, O91, O113, O145, O111, and O26 experimentally inoculated in beef carcass swabs, beef trim, and ground beef.应用多重 PCR 技术检测人工污染牛肉拭子、牛肉切块和绞碎牛肉中属于血清型 O157、O103、O91、O113、O145、O111 和 O26 的产志贺毒素大肠杆菌。
J Food Prot. 2011 Feb;74(2):228-39. doi: 10.4315/0362-028X.JFP-10-386.
4
Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beef.应用多重实时聚合酶链反应检测和分离在-ground 牛肉中的产志贺毒素大肠杆菌血清型 O26、O45、O103、O111、O121 和 O145。
Foodborne Pathog Dis. 2011 May;8(5):601-7. doi: 10.1089/fpd.2010.0773. Epub 2011 Jan 9.
5
Rapid, absolute, and simultaneous quantification of specific pathogenic strain and total bacterial cells using an ultrasensitive dual-color flow cytometer.利用超灵敏双色流式细胞仪快速、绝对且同时定量特定病原菌株和总细菌细胞。
Anal Chem. 2010 Feb 1;82(3):1109-16. doi: 10.1021/ac902524a.
6
Quantitative isolation efficiency of O26, O103, O111, O145 and O157 STEC serotypes from artificially contaminated food and cattle faeces samples using a new isolation protocol.使用一种新的分离方案从人工污染的食品和牛粪样本中定量分离O26、O103、O111、O145和O157 STEC血清型的效率
J Appl Microbiol. 2008 Jul;105(1):227-35. doi: 10.1111/j.1365-2672.2008.03739.x. Epub 2008 Feb 14.
7
Recovery of GFP-labeled bacteria for culturing and molecular analysis after cell sorting using a benchtop flow cytometer.使用台式流式细胞仪进行细胞分选后，回收用于培养和分子分析的绿色荧光蛋白标记细菌。
Microb Ecol. 2004 Aug;48(2):239-45. doi: 10.1007/s00248-003-1069-9. Epub 2004 May 27.
8
Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting.通过多色荧光流式细胞术和单细胞分选分析细菌功能。
J Microbiol Methods. 2000 Sep;42(1):97-114. doi: 10.1016/s0167-7012(00)00181-0.
9
Microbial analysis at the single-cell level: tasks and techniques.单细胞水平的微生物分析：任务与技术
J Microbiol Methods. 2000 Sep;42(1):3-16. doi: 10.1016/s0167-7012(00)00167-6.
Zotero 插件