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应用流式细胞术快速检测碎牛肉中前 6 种非 O157 产志贺毒素大肠埃希氏菌 O 群。

Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry.

机构信息

Escherichia coli Reference Center, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania, USA.

出版信息

J Clin Microbiol. 2012 Jun;50(6):2137-9. doi: 10.1128/JCM.00137-12. Epub 2012 Apr 4.

DOI:10.1128/JCM.00137-12
PMID:22493328
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3372132/
Abstract

Rapid, sensitive, and highly specific flow-cytometric assays were developed for the detection of the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) O groups in ground beef. The analytical sensitivity of the assays was 2 × 10(3) target cells in a bacterial mixture of 10(5) CFU/ml, and the limit of detection in ground beef was 1 to 10 CFU following 8 h of enrichment. The assays may be utilized for rapid detection of STEC O groups in meat.

摘要

快速、灵敏且高度特异的流式细胞术检测方法被开发出来，用于检测牛肉中排名前六的非 O157 产志贺毒素大肠杆菌（STEC）O 群。该检测方法在细菌混合物中检测 10(5)CFU/ml 的目标细胞时，分析灵敏度为 2×10(3)个细胞，在牛肉中经过 8 小时的富集后，检测限为 1 至 10 CFU。这些检测方法可用于快速检测肉类中的 STEC O 群。

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本文引用的文献

1
Detection of O antigens in Escherichia coli.检测大肠杆菌中的 O 抗原。
Anim Health Res Rev. 2011 Dec;12(2):169-85. doi: 10.1017/S1466252311000193.
2
Detection of Shiga toxin-producing Escherichia coli O26, O45, O103, O111, O113, O121, O145, and O157 serogroups by multiplex polymerase chain reaction of the wzx gene of the O-antigen gene cluster.采用 O 抗原基因簇 wzx 基因的多重聚合酶链反应检测产志贺毒素大肠杆菌 O26、O45、O103、O111、O113、O121、O145 和 O157 血清群。
Foodborne Pathog Dis. 2011 May;8(5):651-2. doi: 10.1089/fpd.2010.0769.
3
Multiplex PCR detection of Shiga toxin-producing Escherichia coli strains belonging to serogroups O157, O103, O91, O113, O145, O111, and O26 experimentally inoculated in beef carcass swabs, beef trim, and ground beef.应用多重 PCR 技术检测人工污染牛肉拭子、牛肉切块和绞碎牛肉中属于血清型 O157、O103、O91、O113、O145、O111 和 O26 的产志贺毒素大肠杆菌。
J Food Prot. 2011 Feb;74(2):228-39. doi: 10.4315/0362-028X.JFP-10-386.
4
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Foodborne Pathog Dis. 2011 May;8(5):601-7. doi: 10.1089/fpd.2010.0773. Epub 2011 Jan 9.
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9
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J Microbiol Methods. 2000 Sep;42(1):3-16. doi: 10.1016/s0167-7012(00)00167-6.