Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
J Clin Microbiol. 2012 Jun;50(6):2092-5. doi: 10.1128/JCM.00108-12. Epub 2012 Apr 4.
Mycobacterial growth in liquid culture can go undetected by automated, nonradiometric growth detection systems. In our laboratory, instrument-negative tubes from the Bactec MGIT 960 system are inspected visually for clumps suggestive of mycobacterial growth, which (if present) are examined by acid-fast smear analysis. A 3-year review demonstrated that ∼1% of instrument-negative MGIT cultures contained mycobacterial growth and that 10% of all cultures yielding mycobacteria were instrument negative. Isolates from instrument-negative MGIT cultures included both tuberculous and nontuberculous mycobacteria.
在液体培养中,分枝杆菌的生长可能无法被自动化、非放射性生长检测系统检测到。在我们的实验室中,Bactec MGIT 960 系统的仪器阴性管会通过肉眼检查是否有提示分枝杆菌生长的团块,如果有,则通过抗酸染色分析进行检查。一项为期 3 年的回顾性研究表明,约 1%的仪器阴性 MGIT 培养物中含有分枝杆菌生长,而所有培养出分枝杆菌的培养物中,有 10%的培养物为仪器阴性。从仪器阴性 MGIT 培养物中分离出的分枝杆菌既有结核分枝杆菌也有非结核分枝杆菌。