胆囊癌中 DNA 修复酶 APEX1/REF1 的研究。

Study of AP endonuclease (APEX1/REF1), a DNA repair enzyme, in gallbladder carcinoma.

机构信息

Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.

出版信息

Anticancer Res. 2012 Apr;32(4):1489-92.

Abstract

AIM

This study investigated the levels of Apurinic/Apyrimidinic Endonuclease (APEX1) in gallbladder carcinoma (CaGB) tissue and co-related these levels with various clinicopathological parameters.

PATIENTS AND METHODS

Twenty cases of CaGB and cholelithiasis were included in the study. Western blot analysis of APEX1 protein was performed using actin as the reference point. Densitometric analysis and the integrated density value (IDV) of APEX1 protein samples were determined. The ratio of IDV of APEX1/actin was determined.

RESULTS

The mean IDV ratio of APEX1 in CaGB was 0.63±0.33 and 0.45±0.19 in cholelithiasis. The mean IDV ratio of a variant of APEX1 (ΔAPEX1) in CaGB was 0.50±0.09, whereas it was 0.40±0.16 in cholelithiasis. Calculating the mean IDV ratio of total APEX (APEX1+ΔAPEX1) in CaGB was 1.13±0.31 whereas in cholelithiasis, 0.85±0.23. The differences were statistically significant (p<0.05).

CONCLUSION

A significant correlation was found between the relative expressions of APEX1 in cancer as compared to that in cholelithiasis patients. There was significant association between APEX1 expression and perineural invasion. A variant of APEX1 correlated with tumor infiltration. Hence APEX1 may be of use as a prognostic marker in patients with CaGB.

摘要

目的

本研究旨在调查胆囊癌（CaGB）组织中嘌呤/嘧啶内切核酸酶（APEX1）的水平，并将其与各种临床病理参数相关联。

患者和方法

本研究纳入了 20 例 CaGB 和胆石症患者。使用肌动蛋白作为参照点，通过 Western blot 分析 APEX1 蛋白。对 APEX1 蛋白样本进行密度分析和积分密度值（IDV）测定。测定 APEX1/肌动蛋白的 IDV 比值。

结果

CaGB 中 APEX1 的平均 IDV 比值为 0.63±0.33，胆石症中为 0.45±0.19。CaGB 中 APEX1 的变异型（ΔAPEX1）的平均 IDV 比值为 0.50±0.09，而胆石症中为 0.40±0.16。计算 CaGB 中总 APEX（APEX1+ΔAPEX1）的平均 IDV 比值为 1.13±0.31，而胆石症中为 0.85±0.23。差异具有统计学意义（p<0.05）。