Improved in vitro light activation and assay systems for two spinach chloroplast enzymes.
作者信息
Schürmann P, Jacquot J P
出版信息
Biochim Biophys Acta. 1979 Aug 15;569(2):309-12. doi: 10.1016/0005-2744(79)90067-6.
PMID:224935
Abstract
An improved system for the in vitro light activation of the chloroplast enzymes fructose-1,6-bisphosphatase and NADP-dependent malate dehydrogenase is described. Through the presence of the monothiol beta-mercaptoethanol in the reaction mixtures the activated forms of the enzymes can be stabilized and their activity determined spectrophotometrically.
摘要
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