内分泌治疗后手术治疗的 ER 阳性乳腺癌中高表达 ER-beta 的预后意义。

Prognostic Significance of High Expression of ER-beta in Surgically Treated ER-Positive Breast Cancer Following Endocrine Therapy.

机构信息

Department of Hospital Pathology, The Catholic University of Korea College of Medicine, Seoul, Korea.

出版信息

J Breast Cancer. 2012 Mar;15(1):79-86. doi: 10.4048/jbc.2012.15.1.79. Epub 2012 Mar 28.

PURPOSE

This study evaluated estrogen receptor (ER)-beta mRNA and ER-beta protein expression and its prognostic implications in hormone receptor-positive breast cancer.

METHODS

Paraffin sections from 139 hormone receptor-positive breast cancer cases were prepared. The expression of ER-beta mRNA and protein were analyzed by branched-chain assay and immunohistochemistry (IHC), respectively.

RESULTS

The Allred score of ER-beta IHC was correlated with smaller tumor size (p=0.043), the Allred score of ER-alpha IHC (p<0.001), and the Allred score of progesterone receptor (PR) IHC (p=0.022) but not with the HER2 IHC score. ER-beta mRNA level was correlated with PR mRNA levels (p<0.001) but not with the Allred score of ER-beta IHC, ER-alpha IHC, and PR IHC, nor with the HER2 IHC score and ER-alpha mRNA level. In survival analysis, high expression of ER-beta mRNA was associated with worse disease-free survival along with poor differentiation, lymph node metastasis and absence of PR protein expression in univariate analysis (p=0.040, p=0.002, p=0.018, and p=0.007, respectively) and multivariate analysis (p=0.044, p=0.002, p=0.035, and p=0.007, respectively).

CONCLUSION

High expression of ER-beta mRNA is an independent predictor of disease recurrence in hormone-receptor-positive breast cancer.

目的

本研究评估了激素受体阳性乳腺癌中雌激素受体-β（ER-β）mRNA 和 ER-β 蛋白的表达及其预后意义。

方法

制备了 139 例激素受体阳性乳腺癌石蜡切片。采用分支链分析法和免疫组织化学（IHC）分别分析 ER-β mRNA 和蛋白的表达。

结果

ER-β IHC 的 Allred 评分与肿瘤体积较小（p=0.043）、ER-α IHC 的 Allred 评分（p<0.001）和孕激素受体（PR）IHC 的 Allred 评分（p=0.022）相关，但与 HER2 IHC 评分无关。ER-β mRNA 水平与 PR mRNA 水平相关（p<0.001），但与 ER-β IHC、ER-α IHC 和 PR IHC 的 Allred 评分以及 HER2 IHC 评分和 ER-α mRNA 水平无关。在生存分析中，与 ER-β IHC、ER-α IHC 和 PR IHC 的 Allred 评分以及 HER2 IHC 评分和 ER-α mRNA 水平相比，高 ER-β mRNA 表达与无病生存较差相关，且与低分化、淋巴结转移和 PR 蛋白缺失相关（p=0.040、p=0.002、p=0.018 和 p=0.007 分别；p=0.044、p=0.002、p=0.035 和 p=0.007 分别）。