Li Hui, Bi Juan, Xu Xi-Ming, Li Feng-Qian
Department of Pharmaceutics, Shanghai Eighth People's Hospital, Shanghai 200235, China.
Yao Xue Xue Bao. 2012 Jan;47(1):131-4.
This study is undertaken to modify the chitosan nanoparticles (CS-NPs) with wheat germ agglutinin (WGA), and investigate the conjugation between WGA-CS-NPs and N-acetylglucosamine (NAG). CS-NPs were prepared by ionotropic gelation process and then conjugated with WGA under the activation of glutaricdialdehyde. The mean diameter of the CS-NPs was approximately 113.5 nm and the poly-dispersity index (PDI) was 0.18. The binding yield of WGA to CS-NPs was comprised between 27.8% and 87.9% depending mostly on the addition of 0.3% (w/v) glutaraldehyde solution. A competitive inhibition experiment of WGA-CS-NPs to bovine submaxillary gland mucin (BSM) was taken to illuminate the binding activity of WGA-CS-NPs to the sugar of N-acetylglucosamine. After the addition of NAG, the binding rates between CS-NPs and BSM almost didn't change, while the binding rates between WGA-CS-NPs and BSM dropped down significantly, which confirmed the specific binding characteristics of WGA to NAG.
本研究旨在用麦胚凝集素（WGA）修饰壳聚糖纳米颗粒（CS-NPs），并研究WGA-CS-NPs与N-乙酰葡糖胺（NAG）之间的结合。通过离子凝胶法制备CS-NPs，然后在戊二醛活化下与WGA结合。CS-NPs的平均直径约为113.5nm，多分散指数（PDI）为0.18。WGA与CS-NPs的结合率在27.8%至87.9%之间，这主要取决于0.3%（w/v）戊二醛溶液的添加量。进行了WGA-CS-NPs对牛颌下腺粘蛋白（BSM）的竞争抑制实验，以阐明WGA-CS-NPs与N-乙酰葡糖胺糖的结合活性。添加NAG后，CS-NPs与BSM之间的结合率几乎没有变化，而WGA-CS-NPs与BSM之间的结合率显著下降，这证实了WGA与NAG的特异性结合特性。
