Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843-3122, United States.
Biomacromolecules. 2011 Sep 12;12(9):3205-12. doi: 10.1021/bm200681m. Epub 2011 Aug 11.
Paclitaxel nanoparticles (PAX NPs) prepared with the size of 110 ± 10 nm and ζ potential of -40 ± 3 mV were encapsulated in synthetic/biomacromolecule shell chitosan, dextran-sulfate using a layer-by-layer self-assembly technique. Zeta potential measurements, analysis of X-ray photoelectron spectroscopy, and scanning electron microscopy confirmed the successful adsorption of each layer. Surface modifications of these core-shell NPs were performed by covalently conjugating with poly(ethylene glycol) (H(2)N-PEG-carboxymethyl, M(w) 3400) and fluorescence labeled wheat germ agglutinin (F-WGA) to build a biocompatible and targeted drug delivery system. 32% of PAX was released from four bilayers of biomacromolecule assembled NPs within 8 h as compared with >85% of the drug released from the bare NPs. Moreover, high cell viability with PEG conjugation and high binding capacity of WGA-modified NPs with Caco-2 cells were observed. This biocompatible and targeted NP-based drug delivery system, therefore, may be considered as a potential candidate for the treatment of colonic cancer and other diseases.
紫杉醇纳米颗粒（PAX NPs）的粒径为 110 ± 10nm，ζ 电位为-40 ± 3mV，采用层层自组装技术将其包封在合成/生物大分子壳聚糖、葡聚糖-硫酸酯中。Zeta 电位测量、X 射线光电子能谱分析和扫描电子显微镜证实了各层的成功吸附。通过共价键合聚乙二醇（H（2）N-PEG-羧甲基，M（w）3400）和荧光标记的麦胚凝集素（F-WGA）对这些核壳 NPs 进行表面修饰，构建了一种具有生物相容性和靶向给药系统的纳米颗粒。与裸 NPs 中 >85%的药物释放相比，四层生物大分子组装 NPs 中释放出 32%的 PAX。此外，观察到 PEG 修饰后细胞存活率高，WGA 修饰后的 NPs 与 Caco-2 细胞的结合能力高。因此，这种基于生物相容性和靶向 NP 的药物递送系统可能被认为是治疗结肠癌和其他疾病的潜在候选药物。
