Hammack L
Biosciences Research Laboratory, USDA-ARS, Fargo, North Dakota 58105.
J Econ Entomol. 1991 Feb;84(1):185-90. doi: 10.1093/jee/84.1.185.
Factor affecting oviposition by screwworm flies, Cochliomyia hominivorax (Coquerel), contacting different host fluids were examined in a laboratory bioassay. Fresh bovine blood, which does not release the attractive odors involved in host finding, nevertheless stimulated as many or more females to oviposit than did the other fluids tested. These other fluids included attractive fluid from screwworm-infected wounds (a favored oviposition site in nature) and cultures of Providencia rettgeri (a bacterium implicated in attractant production). Oviposition did not vary with batch of fresh blood or frozen storage, making blood a useful standard for comparing oviposition rates among studies. Oviposition did vary with the substrate to which the blood was applied, suggesting that an interaction between tactile and chemical stimuli is important for host recognition. Both insemination and darkness during bioassay increased oviposition rates, but the magnitude of these effects was small compared with that due to substrate. Age had no effect for at least 1 wk after females became gravid.
在一项实验室生物测定中，研究了影响嗜人锥蝇（Cochliomyia hominivorax (Coquerel)）接触不同宿主液体时产卵的因素。新鲜牛血不会释放宿主寻找过程中涉及的吸引气味，但与其他测试液体相比，它刺激产卵的雌性数量相同或更多。这些其他液体包括来自感染嗜人锥蝇伤口的吸引性液体（自然界中一个受欢迎的产卵部位）和雷氏普罗威登斯菌（一种与引诱剂产生有关的细菌）培养物。产卵量不会因新鲜血液的批次或冷冻储存而有所不同，这使得血液成为比较不同研究中产卵率的有用标准。产卵量会因血液所施加的底物不同而有所变化，这表明触觉和化学刺激之间的相互作用对于宿主识别很重要。生物测定期间的授精和黑暗环境都会提高产卵率，但与底物因素相比，这些影响的程度较小。雌性怀孕后至少1周内，年龄对产卵量没有影响。
