Zhang Xiang-Gen, Jiang Zheng-Lin, Wang Guo-Hua, Li Yong-Cai, Wang Yong, Li Xia, Shen Hong-Mei
Institute of Nautical Medicine, Nantong University, Nantong 226001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2012 Jan;28(1):42-6.
To investigate the effects of hyperbaric oxygen (HBO) treatment on the activation of astrocytes and the expression of glia-derived neurotrophic factor (GDNF) and nerve growth factor (NGF) in the brain after traumatic brain injury (TBI).
54 male SD rats were randomly divided into three groups (n = 18): sham-operated, TBI and HBO treatment groups. TBI was induced with Feeney's method, bone window was opened without strike on the brain tissue in the sham-operated group. HBO group rats received HBO treatment for 60 min in the hyperbaric chamber containing O2 100% at 3 ATA. When neurological functions were measured 48 h after TBI, rats were decapitated, the brain water content of 18 rats was measured, 18 brains were sliced for the morphological observation after Nissl staining and for the immunohistochemistry staining of astrocyte markers glial fibrillary acidic protein (GFAP), vimentin and S100, and the other 18 brains of injured side were used for Western blot analysis of GDNF and NGF.
HBO treatment reduced the neurological deficit, brain water content and hippocampal neuronal loss. In the observed cortex and hippocampal area astrocytes were activated, the cell number of positive expression of astrocyte markers GFAP, vimentin and S100 was increased, and the expression of GDNF and NGF was elevated after TBI. However, these indices were all enhanced further after the HBO treatment.
It is suggested that HBO may be an effective therapy for TBI and upregulation of the expression of GDNF and NGF may underly the effect of HBO.
探讨高压氧（HBO）治疗对创伤性脑损伤（TBI）后大鼠脑内星形胶质细胞活化及胶质细胞源性神经营养因子（GDNF）和神经生长因子（NGF）表达的影响。
将54只雄性SD大鼠随机分为三组（n = 18）：假手术组、TBI组和HBO治疗组。采用Feeney法制备TBI模型，假手术组仅打开骨窗，不打击脑组织。HBO组大鼠在3个绝对大气压（ATA）、100%氧气的高压舱内接受60分钟的HBO治疗。TBI后48小时测量神经功能，然后断头处死大鼠，测量18只大鼠的脑含水量，取18个脑片进行尼氏染色后的形态学观察及星形胶质细胞标志物胶质纤维酸性蛋白（GFAP）、波形蛋白和S100的免疫组织化学染色，另取18个损伤侧脑用于GDNF和NGF的蛋白质印迹分析。
HBO治疗减轻了神经功能缺损、脑含水量及海马神经元丢失。在观察的皮质和海马区域，星形胶质细胞被激活，星形胶质细胞标志物GFAP、波形蛋白和S100阳性表达的细胞数量增加，TBI后GDNF和NGF的表达升高。然而，HBO治疗后这些指标均进一步增强。
提示HBO可能是治疗TBI的有效方法，GDNF和NGF表达上调可能是HBO发挥作用的机制。
