Department of Neuropharmacology, Institute of Nautical Medicine, Nantong University, Nantong, Jiangsu, China.
J Neurotrauma. 2010 Sep;27(9):1733-43. doi: 10.1089/neu.2009.1175.
This study was designed to evaluate the potential benefits of hyperbaric oxygen (HBO) in the treatment of traumatic brain injury (TBI). The right cerebral cortex of rats was injured by the impact of a 20-g object dropped from a predetermined height. The rats received HBO treatment at 3 ATA for 60 min after TBI. Neurological behavior score, brain water content, neuronal loss in the hippocampus, and cell apoptosis in brain tissue surrounding the primary injury site were examined to determine brain damage severity. Three and six hours after TBI, HBO-treated rats displayed a significant reduction in brain damage. However, by 12 h after TBI, the efficacy of HBO treatment was considerably attenuated. Furthermore, at 24, 48, and 72 h after TBI, the HBO treatment did not show any notable effects. In contrast, multiple HBO treatments (three or five times in all), even when started 48 h after TBI, remarkably reduced neurology deficit scores and the loss of neuronal numbers in the hippocampus. Although multiple treatments started at 48 h significantly improved neurological behaviors and reduced brain injury, the overall beneficial effects were substantially weaker than those seen after a single treatment at 6 h. These results suggest that: (1) HBO treatment could alleviate brain damage after TBI; (2) a single treatment with HBO has a time limitation of 12 h post-TBI; and (3) multiple HBO treatments have the possibility to extend the post-TBI delivery time window. Therefore, our results clearly suggest the validity of HBO therapy for the treatment of TBI.
本研究旨在评估高压氧（HBO）治疗创伤性脑损伤（TBI）的潜在益处。通过将 20 克物体从预定高度掉落撞击大鼠右大脑皮层造成损伤。TBI 后，大鼠接受 3ATA 的 HBO 治疗 60min。通过神经行为评分、脑水含量、海马神经元丢失和原发性损伤部位脑组织细胞凋亡来评估脑损伤严重程度。TBI 后 3 小时和 6 小时，HBO 治疗组大鼠脑损伤明显减轻。然而，TBI 后 12 小时，HBO 治疗效果显著减弱。此外，TBI 后 24、48 和 72 小时，HBO 治疗没有显示出任何显著效果。相比之下，多次 HBO 治疗（总共三次或五次），即使在 TBI 后 48 小时开始，也显著降低了神经缺陷评分和海马神经元数量的丢失。尽管多次治疗（48 小时开始）显著改善了神经行为并减轻了脑损伤，但总体有益效果明显弱于单次治疗 6 小时。这些结果表明：（1）HBO 治疗可减轻 TBI 后的脑损伤；（2）单次 HBO 治疗的时间限制为 TBI 后 12 小时；（3）多次 HBO 治疗有可能延长 TBI 后治疗时间窗。因此，我们的结果清楚地表明 HBO 治疗 TBI 的有效性。
