Department of Anatomy and Cell Biology, University of Cantabria, 39011 Santander, Spain.
Acta Histochem. 2013 Mar;115(2):185-9. doi: 10.1016/j.acthis.2012.03.002. Epub 2012 Apr 10.
This report analyzes the localization of collagen and elastin in the teleost bulbus arteriosus by histochemistry and by transmission electron microscopy. Martin's trichrome staining shows widespread distribution of collagen in the wall of the bulbus. However, Sirius red indicates that collagen is mostly restricted to the valves and to the subepicardial layer. This is confirmed by transmission electron microscopy. Trichrome staining gives false positives that may be related to the chemical characteristics of both matrix components and dyes. By contrast, Sirius red constitutes a highly reliable method to detect collagen distribution. On the other hand, orcein heavily stains the bulbus of all teleosts examined. This includes the bulbus of the Antarctic teleosts, which do not show structurally discernable elastin fibers. In these cases, orcein may be staining non-elastin components, or basic elastin components not assembled into larger units. In the teleost bulbus, accurate identification of collagen and elastin cannot be based solely on histochemistry, but should be accompanied by structural identification of the components under study.
本报告通过组织化学和透射电子显微镜分析了硬骨鱼动脉球中胶原蛋白和弹性蛋白的定位。马氏三色染色显示胶原蛋白广泛分布在动脉球壁中。然而，天狼猩红染色表明胶原蛋白主要局限于瓣膜和心外膜下层。这一结果得到了透射电子显微镜的证实。三色染色会产生假阳性，这可能与基质成分和染料的化学特性有关。相比之下，天狼猩红染色是一种高度可靠的方法来检测胶原蛋白的分布。另一方面，地衣红强烈染色所有检查过的硬骨鱼的动脉球。这包括没有结构上可识别的弹性纤维的南极硬骨鱼的动脉球。在这些情况下，地衣红可能是在染色非弹性蛋白成分，或者是尚未组装成更大单元的基本弹性蛋白成分。在硬骨鱼的动脉球中，仅基于组织化学不能准确识别胶原蛋白和弹性蛋白，而应该伴随着对所研究成分的结构鉴定。
