利用原位杂交技术将人类1号和19号染色体携带的标记物GPI、APOE和ENO1定位到猪的6号染色体上。

Localization on pig chromosome 6 of markers GPI, APOE, and ENO1, carried by human chromosomes 1 and 19, using in situ hybridization.

作者信息

Yerle M, Gellin J, Dalens M, Galman O

机构信息

I.N.R.A., Centre de Recherches de Toulouse, Laboratoire de Génétique Cellulaire Castanet-Tolosan France.

出版信息

Cytogenet Cell Genet. 1990;54(1-2):86-91. doi: 10.1159/000132966.

DOI:10.1159/000132966

PMID:2249484

Abstract

In the pig, the linkage group around the halothane gene (HAL), composed of S-GPI-HAL-H-A1BG-PGD, has been assigned to bands p1.2----q2.2 of chromosome 6. In man, ENO1-PGD and APOE-GPI constitute two syntenic groups situated on different chromosomes (1 and 19, respectively). Since GPI and PGD are linked in the pig, we have hybridized the human cDNA probes for ENO1 and APOE to pig chromosomes. These markers were assigned to pig chromosome 6, in the q2.2----q2.4 and cen----q2.1 regions, respectively, using in situ hybridization. Since GPI and APOE are situated in the same region, we combined the use of high resolution chromosome analysis and in situ hybridization to give a more precise localization in the q1.2 and q1.2----q2.1.2 regions of chromosome 6. A possible linear order of these genes is proposed.

摘要

在猪中，围绕氟烷基因（HAL）的连锁群，由S - GPI - HAL - H - A1BG - PGD组成，已被定位到6号染色体的p1.2----q2.2带。在人类中，ENO1 - PGD和APOE - GPI构成两个分别位于不同染色体（1号和19号）上的同线群。由于GPI和PGD在猪中是连锁的，我们已将人类ENO1和APOE的cDNA探针与猪染色体杂交。使用原位杂交技术，这些标记分别被定位到猪6号染色体的q2.2----q2.4和cen----q2.1区域。由于GPI和APOE位于同一区域，我们结合使用高分辨率染色体分析和原位杂交技术，以在6号染色体的q1.2和q1.2----q2.1.2区域进行更精确的定位。本文提出了这些基因可能的线性排列顺序。