猪基因组的初步连锁图谱显示基因重组率较低。
A primary linkage map of the porcine genome reveals a low rate of genetic recombination.
作者信息
Ellegren H, Chowdhary B P, Johansson M, Marklund L, Fredholm M, Gustavsson I, Andersson L
机构信息
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala.
出版信息
Genetics. 1994 Aug;137(4):1089-100. doi: 10.1093/genetics/137.4.1089.
A comprehensive genetic linkage map of the porcine genome has been developed by typing 128 genetic markers in a cross between the European Wild Boar and a domestic breed (Large White). The marker set includes 68 polymerase chain reaction-formatted microsatellites, 60 anchored reference markers informative for comparative mapping and 47 markers which have been physically assigned by in situ hybridization. Novel multipoint assignments are provided for 54 of the markers. The map covers about 1800 cM, and the average spacing between markers is 11 cM. We used the map data to estimate the genome size in pigs, thereby addressing the total recombination distance in a third mammalian species. A sex-average genome length of 1873 +/- 139 cM was obtained by comparing the recombinational and physical distances in defined regions of the genome. This is strikingly different from the length of the human genome (3800-4000 cM) and is more similar to the mouse estimate (1600 cM). The recombination rate in females was significantly higher than in males.
通过对欧洲野猪与一个家猪品种（大白猪）杂交后代中的128个遗传标记进行分型，构建了一张全面的猪基因组遗传连锁图谱。该标记集包括68个经聚合酶链反应格式化的微卫星、60个对比较作图有信息价值的锚定参考标记以及47个已通过原位杂交进行物理定位的标记。为其中54个标记提供了新的多点定位。该图谱覆盖约1800厘摩，标记间的平均间距为11厘摩。我们利用图谱数据来估计猪的基因组大小，从而确定第三个哺乳动物物种的总重组距离。通过比较基因组特定区域的重组距离和物理距离，得到了性别平均基因组长度为1873±139厘摩。这与人类基因组长度（3800 - 4000厘摩）显著不同，而与小鼠的估计值（1600厘摩）更为相似。雌性的重组率显著高于雄性。
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