一株嗜光细菌 Rubrivivax benzoatilyticus JA2 分解 L-苯丙氨酸和生产 L-苯乳酸。
L-Phenylalanine catabolism and L-phenyllactic acid production by a phototrophic bacterium, Rubrivivax benzoatilyticus JA2.
机构信息
Department of Plant Sciences, University of Hyderabad, Hyderabad 500046, India.
出版信息
Microbiol Res. 2012 Oct 12;167(9):526-31. doi: 10.1016/j.micres.2012.03.001. Epub 2012 Apr 10.
A phototrophic bacterium (Rubrivivax benzoatilyticus JA2) grows at the expense of L-phenylalanine as sole source of nitrogen but not as carbon source. Near stoichiometric yields of L-phenylpyruvic acid (0.4 mM) and L-phenyllactate (0.4 mM) were observed from L-phenylalanine (0.9 mM consumed). Aminotransfarase and dehydrogenase activities involved in the formation of L-phenylpyruvic acid and L-phenyllactate were demonstrated unequivocally in Rubrivivax benzoatilyticus JA2. Growth conditions and carbon sources had an influence on L-phenyllactate production. The process yielded a maximum of 0.92 mM L-phenyllactate from L-phenylalanine (1 mM) when fructose served as carbon source for R. benzoatilyticus JA2.
一种好氧光合细菌（Rubrivivax benzoatilyticus JA2）可以利用 L-苯丙氨酸作为唯一氮源进行生长，但不能作为碳源。从 0.9mM 的 L-苯丙氨酸（消耗）中观察到 L-苯丙酮酸（0.4mM）和 L-苯乳酸（0.4mM）近乎化学计量的产率。Rubrivivax benzoatilyticus JA2 中明确证明了形成 L-苯丙酮酸和 L-苯乳酸的氨基转移酶和脱氢酶活性。生长条件和碳源对 L-苯乳酸的生产有影响。当果糖作为 R. benzoatilyticus JA2 的碳源时，该过程从 1mM 的 L-苯丙氨酸中产生了最多 0.92mM 的 L-苯乳酸。
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