多基因座可变数目串联重复分析方案用于猫衣原体基因分型：与多位点序列分型的比较。
Multilocus variable-number tandem-repeat analysis scheme for chlamydia felis genotyping: comparison with multilocus sequence typing.
机构信息
Bacterial Zoonoses Unit, French Agency for Food, Environmental & Occupational Health Safety (ANSES), Maisons-Alfort, France.
出版信息
J Clin Microbiol. 2012 Jun;50(6):1860-6. doi: 10.1128/JCM.00417-12. Epub 2012 Apr 11.
Chlamydia felis is an important ocular pathogen in cats worldwide. A multilocus variable-number tandem-repeat analysis (MLVA) system for the detection of tandem repeats across the whole genome of C. felis strain Fe/C-56 was developed. Nine selected genetic loci were tested by MLVA in 17 C. felis isolates, including the C. felis Baker vaccine strain, and 122 clinical samples from different geographic origins. Analysis of the results identified 25 distinct C. felis MLVA patterns. In parallel, a recently described multilocus sequence typing scheme for the typing of Chlamydia was applied to 13 clinical samples with 12 different C. felis MLVA patterns. Rare sequence differences were observed. Thus, the newly developed MLVA system provides a highly sensitive high-resolution test for the differentiation of C. felis isolates from different origins that is suitable for molecular epidemiological studies.
猫衣原体是一种全球性的重要眼部病原体。本研究开发了一种用于检测猫衣原体菌株 Fe/C-56 全基因组中串联重复序列的多位点可变数串联重复分析（MLVA）系统。通过 MLVA 在 17 株猫衣原体分离株（包括猫衣原体贝克疫苗株）和来自不同地理来源的 122 份临床样本中检测了 9 个选定的遗传基因座。结果分析确定了 25 种不同的猫衣原体 MLVA 模式。同时，应用最近描述的用于分型衣原体的多位点序列分型方案对 13 份具有 12 种不同猫衣原体 MLVA 模式的临床样本进行了分析。观察到了罕见的序列差异。因此，新开发的 MLVA 系统为来自不同来源的猫衣原体分离株的分化提供了一种高度敏感的高分辨率检测方法，适用于分子流行病学研究。
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