[A simplified tissue culture technique for rabbit corneal cells].

A simplified technique for short-term culture of pure populations of rabbit corneal epithelial or endothelial cells has been developed. Rabbit corneas were placed on the concave agarose surfaces of a 24 well culture plate, treated briefly with a solution of trypsin and ethylenediamine tetracetic acid, and transferred, epithelial surface or endothelial surface down as needed, to another 24 well culture plate. Within 24 hours, the epithelial cells or endothelial cells grew over the surfaces of the wells. This technique is simple and economical for the initiation of primary culture of rabbit corneal epithelial or endothelial cells for use in various experiments.