用于活细胞成像的荧光标记支化聚合物和温敏纳米粒子。

Fluorescently labeled branched polymers and thermal responsive nanoparticles for live cell imaging.

机构信息

Department of Polymer Chemistry and Biomaterials, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands.

出版信息

Macromol Biosci. 2012 Apr;12(4):465-74. doi: 10.1002/mabi.201100254.

DOI:10.1002/mabi.201100254

PMID:22496042

Abstract

Branched poly(methoxy-PEG acrylate) and thermally responsive poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) are synthesized by RAFT polymerization. After reduction, these polymers are fluorescently labeled by reacting the free thiol groups with N-(5-fluoresceinyl)maleimide. As shown by DLS, the labeled copolymer poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) forms nanoparticles at body temperature (37 °C) due to the presence of the thermosensitive poly(N-isopropylacrylamide). These materials were used as bioprobes for imaging HUVECs in vitro and chick embryo CAM in vivo. Both labeled polymer and nanoparticles are biocompatible and can be used as efficient fluorescent bioprobes.

摘要

通过 RAFT 聚合合成支化聚（甲氧基聚乙二醇丙烯酸酯）和温敏性聚（甲氧基聚乙二醇丙烯酸酯）-嵌段-聚（N-异丙基丙烯酰胺）。还原后，通过将游离巯基与 N-（5-荧光素）马来酰亚胺反应，将这些聚合物进行荧光标记。如 DLS 所示，由于存在温敏性聚（N-异丙基丙烯酰胺），标记的共聚物聚（甲氧基聚乙二醇丙烯酸酯）-嵌段-聚（N-异丙基丙烯酰胺）在体温（37°C）下形成纳米颗粒。这些材料被用作体外成像人脐静脉内皮细胞（HUVECs）和体内鸡胚 CAM 的生物探针。标记的聚合物和纳米颗粒均具有生物相容性，可用作高效荧光生物探针。