Department of Bioengineering, University of California, Los Angeles, CA 90095-1600, USA.
Macromol Biosci. 2012 Jun;12(6):805-11. doi: 10.1002/mabi.201200002. Epub 2012 Apr 11.
The design, synthesis, and self-assembly of the first dual hydrophilic triblock copolypeptide vesicles, R(H)(m)E(n)L(o) and K(P)(m)R(H)(n)L(o), is reported. Variation of the two distinct hydrophilic domains is used to tune cellular interactions without disrupting the self-assembled structure. The aqueous self-assemblies of these triblock copolypeptides in water are characterized using microscopy and DLS. Cell culture studies are used to evaluate cytotoxicity as well as intracellular uptake of the vesicles. The ability of polypeptides to incorporate ordered chain conformations that direct self-assembly, combined with the facile preparation of functional, multiblock copolypeptide sequences of defined lengths, allow the design of vesicles attractive for development as drug carriers.
报道了首例双亲水性三嵌段共聚物囊泡 R(H)(m)E(n)L(o)和 K(P)(m)R(H)(n)L(o) 的设计、合成和自组装。通过改变两个不同的亲水结构域来调节细胞相互作用，而不破坏自组装结构。使用显微镜和 DLS 对这些三嵌段共聚物在水中的水相自组装进行了表征。细胞培养研究用于评估囊泡的细胞毒性和细胞内摄取。多肽能够形成指导自组装的有序链构象的能力，以及制备具有确定长度的功能性多嵌段共聚物序列的简便性，使得这些囊泡成为有吸引力的药物载体开发材料。
