Rodriguez April R, Choe Uh-Joo, Kamei Daniel T, Deming Timothy J
Department of Bioengineering, University of California, Los Angeles, CA, 90095, USA; Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, 90095, USA.
Macromol Biosci. 2015 Jan;15(1):90-7. doi: 10.1002/mabi.201400348. Epub 2014 Oct 16.
We prepared dual hydrophilic triblock copolypeptide vesicles that form both micron and nanometer scale vesicles in aqueous media. The incorporation of terminal homoarginine segments into methionine sulfoxide-based vesicles was found to significantly enhance their cellular uptake compared to a non-ionic control. We also demonstrated that diblock and triblock copolypeptides with similar hydrophobic domains were found to mix well and form vesicle populations with uniform compositions. Blending of amphiphiles in vesicle nanocarriers was found to impart these materials with many advantageous properties, including good cellular uptake while maintaining minimal toxicity, as well as biological responsiveness to promote vesicle disruption and release of encapsulated cargos.
我们制备了双亲水三嵌段共多肽囊泡，其在水性介质中可形成微米级和纳米级的囊泡。与非离子对照相比，发现将末端高精氨酸片段掺入基于甲硫氨酸亚砜的囊泡中可显著增强其细胞摄取。我们还证明，具有相似疏水结构域的二嵌段和三嵌段共多肽能很好地混合并形成组成均匀的囊泡群体。发现在囊泡纳米载体中混合两亲物可赋予这些材料许多有利特性，包括良好的细胞摄取同时保持最小毒性，以及生物响应性以促进囊泡破裂和释放包封的货物。
