Pham Huu T
Biomedicine. 1979 Jun;30(2):121-4.
Drugs or products acting as chemo-modulators were assayed for their effect on the chemotactic activity of human normal neutrophils by a method of direct microscopic observation. Lectins (PHA-P and Con A) have an inhibitory effect at the level of 10 micrograms/ml. Colchicine and Cytochalasin B have also an inhibitory effect at the level of 0.25.10(-6)M/ml and 0.20 micrograms/ml. For the cyclic nucleotides: dibutyryl cAMP and products such as norepinephrine, aminophylline that raise the cellular level of cAMP have an inhibitory effect, and whereas cGMP and carbamylcholine have a stimulatory effect Imidazole and levamisole, have some enhancement of chemotaxis but it is not significant. Lectins by binding to cell surface receptors which assume the recognition of a chemotactic gradient in the environment, colchicine and cytochalasin B by interfering with the cytoskeletal elements (microtubules and microfilaments) cyclic nucleotides involved in the metabolic activity during the cell motility. The data show that the integrity of one of these three sequences is necessary for the normal activity of chemotasxis. Animal and human models (i.e. mutant mouse bg/bg and Chediak-Higashi syndrome) give some clue of their interrelation.
采用直接显微镜观察法，检测了作为化学调节剂的药物或产品对人正常中性粒细胞趋化活性的影响。凝集素（PHA-P和Con A）在10微克/毫升水平具有抑制作用。秋水仙碱和细胞松弛素B在0.25×10⁻⁶摩尔/毫升和0.20微克/毫升水平也具有抑制作用。对于环核苷酸：二丁酰cAMP以及能提高细胞内cAMP水平的去甲肾上腺素、氨茶碱等产品具有抑制作用，而cGMP和氨甲酰胆碱具有刺激作用。咪唑和左旋咪唑对趋化性略有增强，但不显著。凝集素通过与细胞表面受体结合，这些受体负责识别环境中的趋化梯度，秋水仙碱和细胞松弛素B通过干扰细胞骨架成分（微管和微丝），环核苷酸参与细胞运动过程中的代谢活动。数据表明，这三个序列之一的完整性对于趋化性的正常活动是必要的。动物和人类模型（即突变小鼠bg/bg和Chediak-Higashi综合征）提供了它们相互关系的一些线索。
