Hira Steven M, Aledealat Khaled, Chen Kan-Sheng, Field Mark, Sullivan Gerard J, Chase P Bryant, Xiong Peng, von Molnár Stephan, Strouse Geoffrey F
Department of Chemistry and Biochemistry, The Florida State University, Tallahassee, FL 32306-4390, USA.
J Biomed Biotechnol. 2012;2012:492730. doi: 10.1155/2012/492730. Epub 2012 Feb 13.
Sensing biological agents at the genomic level, while enhancing the response time for biodetection over commonly used, optics-based techniques such as nucleic acid microarrays or enzyme-linked immunosorbent assays (ELISAs), is an important criterion for new biosensors. Here, we describe the successful detection of a 35-base, single-strand nucleic acid target by Hall-based magnetic transduction as a mimic for pathogenic DNA target detection. The detection platform has low background, large signal amplification following target binding and can discriminate a single, 350 nm superparamagnetic bead labeled with DNA. Detection of the target sequence was demonstrated at 364 pM (<2 target DNA strands per bead) target DNA in the presence of 36 μM nontarget (noncomplementary) DNA (<10 ppm target DNA) using optical microscopy detection on a GaAs Hall mimic. The use of Hall magnetometers as magnetic transduction biosensors holds promise for multiplexing applications that can greatly improve point-of-care (POC) diagnostics and subsequent medical care.
在基因组水平上检测生物制剂，同时比常用的基于光学的技术（如核酸微阵列或酶联免疫吸附测定（ELISA））提高生物检测的响应时间，是新型生物传感器的一个重要标准。在此，我们描述了通过基于霍尔效应的磁转导成功检测35个碱基的单链核酸靶标，以此模拟致病DNA靶标的检测。该检测平台背景低，靶标结合后信号大幅放大，并且能够区分单个标记有DNA的350 nm超顺磁性珠。在存在36 μM非靶标（非互补）DNA（<10 ppm靶标DNA）的情况下，使用砷化镓霍尔效应模拟物通过光学显微镜检测，在364 pM（每个珠子<2条靶标DNA链）的靶标DNA中证明了对靶标序列的检测。将霍尔磁力计用作磁转导生物传感器有望用于多重应用，这可以极大地改善即时护理（POC）诊断及后续医疗护理。
