Selvarajah Suganya, Chatterji Udayan, Kuhn Richard, Kinney Richard, Vasudevan Subhash G, Gallay Philippe
Department of Immunology & Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA.
Open Virol J. 2012;6:29-37. doi: 10.2174/1874357901206010029. Epub 2012 Mar 29.
The astonishing speed with which Dengue has spread across the world and the severity of its infection make Dengue a prime threat to human life worldwide. Unfortunately, to date there are no effective vaccines or treatments against Dengue. Since only a few assays permit rapid and sensitive detection of Dengue, we developed a specific antigen capture enzyme-linked immunosorbent assay (ELISA) for the abundant structural Dengue-2 capsid protein. We showed that the ELISA allows rapid and sensitive detection of Dengue-2 replication in various cell lines including human and mosquito cells. Using anti-capsid antibodies, we demonstrated that the capsid ELISA is as accurate as other well-characterized Dengue assays such as intracellular FACS staining (IFSA) and fluorescent focus (FFA) assays. The capsid ELISA not only represents a useful tool for in vitro basic research, but it may also represent a valuable diagnostic tool for Dengue infection in patients.
登革热在全球传播的惊人速度及其感染的严重性使其成为全球人类生命的主要威胁。不幸的是，迄今为止，尚无针对登革热的有效疫苗或治疗方法。由于只有少数检测方法能够快速、灵敏地检测登革热，我们针对丰富的登革热2型衣壳结构蛋白开发了一种特异性抗原捕获酶联免疫吸附测定（ELISA）。我们证明，该ELISA能够快速、灵敏地检测包括人类和蚊子细胞在内的各种细胞系中的登革热2型复制。使用抗衣壳抗体，我们证明衣壳ELISA与其他已充分表征的登革热检测方法（如细胞内流式细胞术染色（IFSA）和荧光灶（FFA）检测）一样准确。衣壳ELISA不仅是体外基础研究的有用工具，也可能是诊断登革热感染患者的有价值诊断工具。
