[铜绿假单胞菌噬菌体phiPMG1的特性]

[The properties of the Pseudomonas aeruginosa bacteriophage phiPMG1].

作者信息

Chertkov O V, Chuprov-Netochin R N, Legotskiĭ S V, Sykilinda N N, Shneider M M, Ivanova M A, Pleteneva E A, Shaburova O V, Burkal'tseva M B, Kostriukova E S, Lazarev V N, Kliachko N L, Miroshnikov K A

出版信息

Bioorg Khim. 2011 Nov-Dec;37(6):807-14. doi: 10.1134/s1068162011060057.

DOI:10.1134/s1068162011060057

PMID:22497079

Abstract

The properties of the isolated Pseudomonas aeruginosa bacteriophage phiPMG1 include the lytic infection cycle, and the formation of a broad halo (semi-transparent zone) around the plaques. We consider phiPMG1 as a potential member of therapeutic cocktails of live phages, and as a source of peptidoglycan and lipopolysaccharide degrading enzymes. Partial sequencing of phiPMG1 genome has revealed high similarity with known temperate P. aeruginosa phage D3. An open reading frame encoding lytic transglycosilase was identified in the genome. This enzyme PMG MUR was obtained in recombinant form, and its activity and substrate specificity has been studied.

摘要

分离出的铜绿假单胞菌噬菌体phiPMG1的特性包括溶菌感染周期，以及在噬菌斑周围形成一个宽晕圈（半透明区）。我们认为phiPMG1是活噬菌体治疗组合的潜在成员，也是肽聚糖和脂多糖降解酶的来源。phiPMG1基因组的部分测序显示与已知的温和铜绿假单胞菌噬菌体D3高度相似。在基因组中鉴定出一个编码溶菌转糖基酶的开放阅读框。这种酶PMG MUR以重组形式获得，并对其活性和底物特异性进行了研究。