Krylov S V, Kropinski A M, Pleteneva E A, Shaburova O V, Burkal'tseva M V, Miroshnikov K A, Krylov V N
Genetika. 2012 Sep;48(9):1057-67.
Results of studying the novel virulent phage phiPMG1 active on Pseudomonas aeruginosa are presented. phiPMG1 was shown to exhibit detectable homology and resemblance in the total genome structure with temperate converting phage D3. Phage phiPMG1 differs from D3 in that it fails to stably lysogenize bacteria and can grow on strains carrying plasmids that cause growth inhibition of phage D3 and some other phages. This significantly diminishes the probability of horizontal gene transfer with phage phiPMG1 and suggests the possible employment of this phage in phage therapy. A comparison of genome structures in phages phiPMG1 and D3 demonstrated not only high homology of 65 genes, but also the presence in the phiPMG1 genome of 16 genes that were not recorded in the files of NCBI database. Apparently, the evolution of genomes in phages of this species is mostly associated with migrations into other species of bacteria and recombinations with phages of other species (for example, F116). Detailed structural analysis a genome region in which the essential nonhomology is exhibited between three D3-like phages (D3, phiPMG1, and PAJU2) revealed that the phiPMG1 genome supposedly is phylogenetically closer than the others to the genome of a hypothetical ancestor phage belonging to this species.
本文展示了对新型毒力噬菌体phiPMG1作用于铜绿假单胞菌的研究结果。phiPMG1在全基因组结构上与温和转化噬菌体D3表现出可检测到的同源性和相似性。噬菌体phiPMG1与D3的不同之处在于，它不能稳定地使细菌溶原化，并且可以在携带导致噬菌体D3和其他一些噬菌体生长抑制的质粒的菌株上生长。这显著降低了噬菌体phiPMG1进行水平基因转移的可能性，并表明该噬菌体在噬菌体治疗中可能的应用。对噬菌体phiPMG1和D3的基因组结构比较表明，不仅有65个基因具有高度同源性，而且在phiPMG1基因组中存在16个未在NCBI数据库文件中记录的基因。显然，该物种噬菌体基因组的进化主要与向其他细菌物种的迁移以及与其他物种噬菌体（例如F116）的重组有关。对三个类D3噬菌体（D3、phiPMG1和PAJU2）之间表现出基本非同源性的基因组区域进行详细结构分析，结果显示phiPMG1基因组在系统发育上可能比其他基因组更接近属于该物种的假想祖先噬菌体的基因组。
