Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Kendall Hall, 129 Main Street, Durham, NH 03824, USA.
J Fish Biol. 2012 Apr;80(5):1300-19. doi: 10.1111/j.1095-8649.2011.03171.x. Epub 2011 Dec 23.
Male and female spiny dogfish Squalus acanthias were collected in the western North Atlantic Ocean in the Gulf of Maine between July 2006 and June 2009. Squalus acanthias ranged from 25 to 102 cm stretch total length and were caught during all months of the year except January. Age estimates derived from banding patterns visible in both the vertebrae and second dorsal-fin spines were compared. Vertebral growth increments were visualized using a modified histological staining technique, which was verified as appropriate for obtaining age estimates. Marginal increment analysis of vertebrae verified the increment periodicity, suggesting annual band deposition. Based on increased precision and accuracy of age estimates, as well as more biologically realistic parameters generated in growth models, the current study found that vertebrae provided a more reliable and accurate means of estimating age in S. acanthias than the second dorsal-fin spine. Age estimates obtained from vertebrae ranged from <1 year-old to 17 years for male and 24 years for female S. acanthias. The two-parameter von Bertalanffy growth model fit to vertebrae-derived age estimates produced parameters of L∞ = 94·23 cm and k = 0·11 for males and L∞ = 100·76 cm and k = 0·12 for females. While these growth parameters differed from those previously reported for S. acanthias in the western North Atlantic Ocean, the causes of such differences were beyond the scope of the current study and remain to be determined.
在 2006 年 7 月至 2009 年 6 月期间，在北大西洋的缅因湾地区采集了雄性和雌性棘鲨（Squalus acanthias）。棘鲨全长 25 至 102 厘米，在一年中的所有月份都有捕获，除了 1 月。对来自脊椎和第二背鳍刺上可见的条带模式的年龄估计进行了比较。使用改良的组织学染色技术观察到脊椎的生长增量，该技术被证明适合获取年龄估计。脊椎的边缘增量分析验证了增量的周期性，表明每年都有带沉积。基于年龄估计的精度和准确性的提高，以及生长模型中生成的更符合生物学现实的参数，本研究发现，与第二背鳍刺相比，脊椎提供了一种更可靠和准确的估计棘鲨年龄的方法。从脊椎获得的年龄估计值从雄性的 <1 岁到 17 岁，以及雌性的 24 岁不等。基于脊椎获得的年龄估计值的双参数 von Bertalanffy 生长模型产生的参数为雄性的 L∞=94·23 厘米和 k=0·11，以及雌性的 L∞=100·76 厘米和 k=0·12。虽然这些生长参数与以前在北大西洋报道的棘鲨的生长参数不同，但造成这种差异的原因超出了本研究的范围，有待确定。
