Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, ROC.
J Hazard Mater. 2012 Jun 15;219-220:43-9. doi: 10.1016/j.jhazmat.2012.03.035. Epub 2012 Mar 20.
While phytoremediation has been considered as an in situ bioprocess to remediate environmental contaminants, the application of functional endophytic bacteria within plants remains a potential strategy that could enhance the plants' efficiency in phytoremediation. In this study, 219 endophytes were isolated from plants that are predominantly located in a constructed wetland, including reed (Phragmites australis) and water spinach (Ipomoea aquatica). Twenty-five strains of the isolated endophytes utilize aromatic compounds as sole carbon source; Achromobacter xylosoxidans strain F3B was chosen for the in planta studies using the model plant Arabidopsis thaliana. Phylogenetic analysis indicated that those endophytic isolates of A. xylosoxidans formed a cluster within its species, and a specific real-time PCR detection method was developed for confirming the stability of the isolates in plants. In the presence of either catechol or phenol, inoculation of A. thaliana with F3B could extend into the root lengths and fresh weights to promote pollutants removal rates. These results demonstrate the potential of the endophytic F3B strain for helping plants to tolerate stress from aromatic compounds and to improve phytoremediation of phenolic pollutants.
虽然植物修复被认为是一种原位生物过程，可用于修复环境污染物，但在植物内应用功能内生细菌仍然是一种潜在的策略，可以提高植物在植物修复中的效率。在这项研究中，从主要位于人工湿地的植物中分离出了 219 种内生菌，包括芦苇（Phragmites australis）和空心菜（Ipomoea aquatica）。从分离出的内生菌中，有 25 株可以利用芳香族化合物作为唯一碳源；选择阿克曼氏菌（Achromobacter xylosoxidans）F3B 菌株进行模式植物拟南芥的体内研究。系统发育分析表明，这些内生菌分离株在其种内形成一个聚类，并且开发了一种特定的实时 PCR 检测方法来确认分离株在植物中的稳定性。在存在儿茶酚或苯酚的情况下，用 F3B 接种拟南芥可以延长根长和鲜重，以促进污染物去除率。这些结果表明，内生 F3B 菌株具有帮助植物耐受芳香族化合物的压力和提高酚类污染物植物修复的潜力。
