Characterization of endophytic bacteriome diversity and associated beneficial bacteria inhabiting a macrophyte .

INTRODUCTION

The endosphere of a plant is an interface containing a thriving community of endobacteria that can affect plant growth and potential for bioremediation. is an aquatic macrophyte, adapted to estuarine and freshwater ecosystems, which harbors a diverse bacterial community. Despite this, we currently lack a predictive understanding of how taxonomically structure the endobacterial community assemblies across distinct habitats (root, stem, and leaf).

METHODS

In the present study, we assessed the endophytic bacteriome from different compartments using 16S rRNA gene sequencing analysis and verified the plant beneficial potential of isolated bacterial endophytes of .

RESULTS AND DISCUSSION

Plant compartments displayed a significant impact on the endobacterial community structures. Stem and leaf tissues were more selective, and the community exhibited a lower richness and diversity than root tissue. The taxonomic analysis of operational taxonomic units (OTUs) showed that the major phyla belonged to Proteobacteria and Actinobacteriota (> 80% in total). The most abundant genera in the sampled endosphere was in both stem and leaf samples. Members of the family Rhizobiaceae, such as in both stem and leaf samples. Members of the family Rhizobiaceae, such as were mainly associated with leaf tissue, whereas the genera and from the families Nannocystaceae and Nitrospiraceae, respectively, were statistically significantly associated with root tissue. were putative keystone taxa of stem tissue. Most of the endophytic bacteria isolated from showed plant beneficial effects known to stimulate plant growth and induce plant resistance to stresses. This study provides new insights into the distribution and interaction of endobacteria across different compartments of Future study of endobacterial communities, using both culture-dependent and -independent techniques, will explore the mechanisms underlying the wide-spread adaptability of to various ecosystems and contribute to the development of efficient bacterial consortia for bioremediation and plant growth promotion.