Fan Di, Schwinghamer Timothy, Liu Shuaitong, Xia Ouyuan, Ge Chunmei, Chen Qun, Smith Donald L
School of Biology, Food and Environment, Hefei University, Hefei, China.
Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
Front Plant Sci. 2023 Jun 19;14:1176648. doi: 10.3389/fpls.2023.1176648. eCollection 2023.
The endosphere of a plant is an interface containing a thriving community of endobacteria that can affect plant growth and potential for bioremediation. is an aquatic macrophyte, adapted to estuarine and freshwater ecosystems, which harbors a diverse bacterial community. Despite this, we currently lack a predictive understanding of how taxonomically structure the endobacterial community assemblies across distinct habitats (root, stem, and leaf).
In the present study, we assessed the endophytic bacteriome from different compartments using 16S rRNA gene sequencing analysis and verified the plant beneficial potential of isolated bacterial endophytes of .
Plant compartments displayed a significant impact on the endobacterial community structures. Stem and leaf tissues were more selective, and the community exhibited a lower richness and diversity than root tissue. The taxonomic analysis of operational taxonomic units (OTUs) showed that the major phyla belonged to Proteobacteria and Actinobacteriota (> 80% in total). The most abundant genera in the sampled endosphere was in both stem and leaf samples. Members of the family Rhizobiaceae, such as in both stem and leaf samples. Members of the family Rhizobiaceae, such as were mainly associated with leaf tissue, whereas the genera and from the families Nannocystaceae and Nitrospiraceae, respectively, were statistically significantly associated with root tissue. were putative keystone taxa of stem tissue. Most of the endophytic bacteria isolated from showed plant beneficial effects known to stimulate plant growth and induce plant resistance to stresses. This study provides new insights into the distribution and interaction of endobacteria across different compartments of Future study of endobacterial communities, using both culture-dependent and -independent techniques, will explore the mechanisms underlying the wide-spread adaptability of to various ecosystems and contribute to the development of efficient bacterial consortia for bioremediation and plant growth promotion.
植物的内圈是一个界面，其中包含一个繁荣的内生细菌群落，该群落可影响植物生长及生物修复潜力。[植物名称]是一种水生大型植物，适应河口和淡水生态系统，其栖息着多样的细菌群落。尽管如此，目前我们仍缺乏对[植物名称]如何在不同生境（根、茎和叶）中对内生细菌群落组装进行分类构建的预测性理解。
在本研究中，我们使用16S rRNA基因测序分析评估了来自不同区室的内生细菌群落，并验证了从[植物名称]分离出的内生细菌对该植物的有益潜力。
植物区室对内生细菌群落结构有显著影响。茎和叶组织的选择性更强，且该群落的丰富度和多样性低于根组织。对操作分类单元（OTU）的分类分析表明，主要门属于变形菌门和放线菌门（总计>80%）。在采样的内圈中，茎和叶样本中最丰富的属均为[属名]。根瘤菌科成员，如[菌名]，主要与叶组织相关，而分别来自囊菌科和硝化螺旋菌科的[属名1]和[属名2]在统计学上与根组织显著相关。[菌名]是茎组织的假定关键分类群。从[植物名称]分离出的大多数内生细菌显示出已知的对该植物有益的作用，可刺激植物生长并诱导植物对胁迫的抗性。本研究为[植物名称]不同区室内内生细菌的分布和相互作用提供了新见解。未来使用依赖培养和不依赖培养技术对内生细菌群落进行的研究，将探索[植物名称]对各种生态系统广泛适应性的潜在机制，并有助于开发用于生物修复和促进植物生长的高效细菌联合体。
